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5139942 |
Method for producing the Nde I restriction endonuclease and methylase
The present invention is directed to a method for cloning and producing the Nde I restriction endonuclease by 1) introducing the restriction endonuclease gene from Neisseria denitrificans into a...
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5137823 |
Method for producing the Bamhi restriction endonuclease and methylase
A two-step method for cloning the BamHI restriction modification system is provided which comprises introducing the BamHI modification or methylase gene into a host cell and expressing the gene to...
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5134067 |
Type II restriction endonuclease RleAI
The type II restriction endonuclease RleAI has the following recognition sequence: ##STR1## cleaves DNA at the cleavage site indicated by the arrows, and is preferably obtainable from...
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5134068 |
Type II restriction endonucleae MCRI
Type II restriction endonuclease McrI is disclosed. This endonuclease has recognition sequence: ##STR1## wherein R is G or A and Y is C or T, and the cleavage site indicated by the arrows. The...
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5134069 |
Type II restriction endonuclease SgrAI
The type III restriction endonuclease SgrAI has the following recognition sequence: ##STR1## cleaves DNA at the cleavage site indicated by the arrows, and is preferably obtainable from...
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5120651 |
Restriction enzyme AgeI and process for producing same
A restriction enzyme capable of recognizing and cleaving a DNA sequence at a position indicated by the arrows: t'-A↓CCGGT-3' 3'-TGGCC↑A -5'.
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5116742 |
RNA ribozyme restriction endoribonucleases and methods
New RNA endoribonuclease ribozymes are found with new conditions to prevent mismatch cleavage and able to cleave RNA after 6 different sets of ribonucleotide 4 base sequences.
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5106950 |
Polypeptide-labeled analyte analog for carrying out an immunoassay
A polypeptide-labeled analyte analog for use in an immunoassay is prepared which is capable of binding with a polypeptide partner to provide enzymatic activity such as a ribonuclease or...
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5100793 |
Method for producing the AseI restriction endonuclease and methylase
The present invention is directed to a method for cloning and producing the AseI restriction endonuclease by 1) introducing the restriction endonuclease gene from A. serpens ATCC 12638 into a host...
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5075232 |
Method for producing the NLAVI restriction endonuclease and methylase
The present invention is directed to a method for cloning and producing the NlaIV restriction endonuclease by 1) introducing the restriction endonuclease gene from N. lactamica NRCC 2118 into a...
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5073486 |
Assay for Mycoplasma fermentans
An assay for detecting the presence of Mycoplasma fermentans is disclosed, along with a diagnostic kit that uses the assay. The presence of Mycoplasma fermentans is indicated by the presence of the...
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5061626 |
Antigenic anarogues of platelet activating factor
The invention concerns antigens for the production of antibodies to Platelet Activating Factor (PAF). The antigens are PAF analogues of formula (I) ##STR1## wherein X comprises a high molecular...
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5061628 |
Restriction endonuclease FseI
A novel Type II restriction endonuclease isolated from Frankia species DDB13250120 is provided. The enzyme, designated FseI, recognizes the octanucleotide sequence ##STR1## and cleaves Adenovirus 2...
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5053330 |
Method for producing the MwoI restriction endonuclease and methylase
The present invention is directed to a method for cloning and producing the MwoI restriction endonuclease by 1) introducing the restriction endonuclease gene from M. wolfei into a host whereby the...
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5049501 |
Production method for PvuI restriction endonuclease
A recombinant vector comprising an incorporated chromosome DNA fragment containing a PvuI restriction enconuclease gene derived from Proteus vulgaris, a host transformed with the recombinant vector...
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5034325 |
5'-phosphodiesterase enzyme preparation and method for its production
This invention relates to a 5'-phosphodiesterase enzyme preparation which exhibits excellent storage stability. It is obtained by a special extraction process from rapidly proliferating parts of...
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5030569 |
Method for producing the AFL II restriction endonuclease and methylase
The present invention is directed to a method for cloning and producing the Afl II restriction endonuclease by 1) introducing the restriction endonuclease gene from Anabaena flos-aquae into a host...
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5004691 |
Method for producing the ACCI restriction endonuclease and methylase
The present invention is directd to a method for cloning and producing the AccI restriction endonuclease by (1) introducing the restriction endonuclease gene from into a host whereby the...
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5002882 |
Method for producing the XmaI restriction endonuclease and methylase
The present invention is directed to a method for cloning and producing the XmaI restriction endonuclease by (1) introducing the restriction endonuclease gene from X. malvacaerum into a host...
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4999294 |
Method for producing the FokI restriction endonuclease and methylase
The present invention is directed to a method for cloning and producing the FokI restriction endonuclease by (1) introducing the restriction endonuclease gene from Flavobacterium okeanokoites IFO...
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4999293 |
Method for producing the HhaI restriction endonuclease and methylase
The present invention is directed to a method for cloning and producing the HhaI restriction endonuclease by (1) introducing the restriction endonuclease gene from Haemophilus haemolyticus ATCC...
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4996151 |
Method for producing the Eag I restriction endonuclease and methylase
The present invention is directed to a method for cloning and producing the Eag I restriction endonuclease by (1) introducing the restriction endonuclease gene from E. agglomerans ATCC into a host...
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4994370 |
DNA amplification technique
A modification of the polymerase chain reaction (PCR) technique is described. The method allows the amplification of regions of DNA flanking a single region of known sequence, in contrast to...
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4988620 |
Method for producing the FnuDI restriction endonuclease and methylase
The present invention is directed to a method for cloning and producing the FnuDI restriction endonuclease by (1) introducing the restriction endonuclease gene from F. nucleatum D into a host...
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4987074 |
Method for producing the HgiAI restriction endonuclease and methylase
The present invention is directed to a method for cloning and producing the HgiAI restriction endonuclease by (1) introducing the restriction endonuclease gene from Herpetosiphon giganteus into a...
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4985243 |
Composition and method for protecting against diseases caused by microorganisms
A vaccine for protecting against a disease caused by a microorganism which does not synthesize nucleic acid precursors such as a Micoplasma organism, which contains nuclease and/or a nuclease...
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4983522 |
Method for producing the HinPI restriction endonuclease and methylase
The present invention is directed to a method for cloning and producing the HinPI restriction endonuclease by (1) introducing the restriction endonuclease gene from Haemophilus influenza P1 into a...
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4983542 |
Method for producing the XbaI restriction endonuclease and methylase
The present invention is directed to a method for cloning and producing the XbaI restriction endonuclease by (1) introducing the restriction endonuclease gene from Xanthomonas badrii into a host...
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4975376 |
Class II restriction endonuclease KspI and a process for obtaining it
The present invention provides a restriction endonuclease which recognizes palindromic sequences ##STR1## where C* is methylated, and cleaves these sequences at the position indicated by the...
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4970149 |
Class II restriction endonuclease Ksp632I, a process for obtaining it and the use thereof
The present invention provides a restriction endonuclease, characterized by the recognition sequence: 5'-CTCTTCN↓NNN-N-3' 3'-GAGAAGN-NNN↑N-5' and the cleavage position defined by the...
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4966849 |
CDNA and genes for human angiogenin (angiogenesis factor) and methods of expression
DNA sequences encoding a protein having angiogenic activity and mutated DNA sequences encoding a protein having decreased angiogenic activity are disclosed. Expression vectors containing these...
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4935357 |
Universal restriction endonuclease
A universal restriction endonuclease for cleaving a target DNA at a predetermined site. The universal restriction endonuclease utilizes a tailored oligodeoxynucleotide adapter in conjunction with a...
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4935367 |
Novel restriction endonuclease
A new restriction enzyme, Mfe I, has been discovered. Mfe I recognizes the sequence CAATTG and cuts at the recognition sequence C'AATTG and generates compatible cohesive ends with EcoRI cleaved...
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4900673 |
Mutant human angiogenin (angiogenesis factor with superior angiogenin activity) genes therefor and methods of expression
Site-specific mutagenesis of a gene for angiogenin producing DNA sequences encoding mutant proteins having increased angiogenic activity are disclosed. Expression vectors containing these sequences...
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4886756 |
Novel restriction endonuclease SplI and process for the production of the same
A novel restriction endonuclease SplI which has the following physicochemical properties: (1) recognizing the following base sequences in double-stranded deoxyribonucleic acid ##STR1## and...
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4871664 |
Type II restriction endonuclease Dsa I with process for obtaining it and the use thereof
The present invention provides a type II restriction endonuclease isolated from Dactylococcopsis salina. The endonuclease is known as Dsa I, and recognizes the following nucleotide sequence and has...
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4863858 |
Restriction endonucleas DRA III
The present invention teaches a restriction endonuclease which recognizes and cleaves the following palendromic sequence of nucleic acids at that point indicated by the arrows: ##STR1## One source...
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4840901 |
Restriction endonuclease Dra II
The present invention teaches a restriction endonuclease which recognizes and cleaves palindromic sequence 5'Pu G G N C C Py 3' 3'Py C C N G G Pu 5' as well as a process for obtaining this...
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4833082 |
New restriction enzyme and process for producing the same
A restriction enzyme, ApaLI, which recognizes the base sequence in a double-stranded deoxyribonucleic acid molecule as shown below, and cleaves it at the arrow-marked sites, ##STR1## wherein A, G,...
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4797361 |
Microorganism and process
This invention includes a process for producing and using increased quantities of extracellular glycoprotein (particularly cellulolytic) enzymes by permitting the enzyme-producing microbial strain...
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4785080 |
Labeled analytes
A highly sensitive, immunoassay method for determining the amount of an analyte in a sample containing a known analyte in an unknown concentration is provided. Sample; a polypeptide-labeled analog...
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4746609 |
Restriction endonuclease cleaving palindromic DNA
The present invention teaches restriction endonuclease Asp 718 which cleaves DNA in the palindromic recognition sequence at the sites indicated by the arrow: ##STR1## The endonuclease, obtained...
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4724209 |
Process for producing restriction enzyme
A process for producing a restriction endonuclease capable of recognizing the nucleotide sequence ##STR1## (wherein A, C, G and T represent adenosine, cytidine, guanosine and thymidine,...
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4693978 |
Type II restriction endonuclease MaeI, a process for obtaining it and the use thereof
The present invention provides a restriction endonuclease, characterized by the palindromic recognition sequence: ##STR1## and the cleavage position defined by the arrows. The present invention...
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4693980 |
New type II restriction endonuclease mae III, a process for obtaining it and the use thereof
The present invention provides a restriction endonuclease, characterized by the palindromic recognition sequence: ##STR1## and the cleavage position defined by the arrows. The present invention...
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4588689 |
Restriction endonuclease XcyI
A novel restriction endonuclease designated XcyI recognizes and cleaves the sequence 5'-C↓CCGGG-3', where the arrow indicates the cleavage site. The enzyme may be obtained from Xanthamonas...
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4542099 |
Method for manufacturing restriction enzymes from Bifidobacteria
This invention relates to a method for manufacturing restriction enzymes, which comprises cultivating a restriction enzyme-producing strain belonging to Bifidobacterium and recovering the...
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4495279 |
Isoelectric focussing-polynucleotide/polyacrylamide gel electrophoresis
An electrophoresis system which provides enhanced resolution and ability to identify nucleases through use of a two-dimensional technique involving isoelectric focussing of tube gels followed by...
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4430432 |
Endo-deoxyribonuclease and process for the production thereof
A novel Endo-deoxyribonuclease A (Endo-DNase A) which has the substrate specificity of recognizing specific base sequence in double-stranded deoxyribonucleic acid (DNA) molecules and cleaving the...
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4342833 |
Immobilized restriction endonucleases
Restriction endonucleases are immobilized by chemically bonding the restriction endonucleases to water-insoluble matrices. The immobilized restriction endonucleases have enhanced stability and can...
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