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5723326 |
Genome coding phytolacca antiviral protein and a recombinant expression vector therefor
The present inventors discovered a novel genome coding Phytolacca insularis antiviral protein(PIP) isolated from Phytolacca insularis Nakai; and developed a recombinant vector for said PIP genome...
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5721126 |
Method for cloning and producing the SCaI restriction endonuclease in E. coli
The present invention relates to isolated DNA coding for the restriction endonuclease SCaI as well as to a method for cloning methylase genes from Streptomyces into E. coli by a modification of the...
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5719028 |
Cleavase fragment length polymorphism
A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is...
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5686286 |
hPDE IV-C: a novel human phosphodiesterase IV isozyme
This invention relates to a novel nucleic acid sequence encoding a novel human phosphodiesterase IV (hPDE IV) isozyme. It also relates to a polypeptide encoded by such sequence. This invention also...
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5677289 |
Method of cleaving specific strands of RNA and medical treatments thereby
A method of using a chimeric molecule made up of an antisense oligonucleotide attached to a 2',5'-oligoadenylate molecule to specifically cleave a sense strand of RNA, wherein the antisense...
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5670359 |
Cloned NsiI restriction-modification system
The present invention discloses the cloning and expression in Escherichia coli of the NsiI restriction-modification system from Neisseria sicca, utilizing a two step protocol. Initial protection of...
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5670366 |
Recombinant DNA sequences encoding phospholipase
Substantially isolated and purified eukaryotic nucleotide sequences encoding for plant phospholipases (e.g., phospholipase D) are disclosed which can be inserted into an expression vector and used...
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5663067 |
Method for cloning and producing the SapI restriction endonuclease in E. coli
The present invention relates to recombinant DNA which encodes the SapI restriction endonuclease and modification methylase, and the production of SapI restriction endonuclease from the recombinant...
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5646026 |
Ribosome-inactivating proteins, inactive precursor forms thereof, a process for making and a method of using
The present invention is directed to a ribosome inactivating proteins. The proteins are characterized by being in a single chain proRIP inactive form that can be converted into an active form by...
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5637476 |
Method for cloning and producing the SFII restriction endonuclease and methylase
The present invention is directed to a method for cloning and producing the SfiI restriction endonuclease by 1) introducing the restriction endonuclease gene from S. fimbriatus into a host whereby...
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5635381 |
Agrobacterium bacteria capable of site-specific recombination
The invention provides Agrobacterium strains capable of producing a cite-specific recombinase capable of effecting site-specific recombination of a first and a second recombination site in...
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5633438 |
Microspore-specific regulatory element
A novel DNA regulatory element that confers microspore-specific gene expression has been discovered, isolated, and characterized. The microspore-specific regulatory element can be used to control...
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5616484 |
Cloning and expression of the ApaLI restriction endonuclease
The present invention relates to a method of cloning ApaLI methylase gene (apaLIM) and ApaLI endonuclease gene (apaLIR) from Acetobacter pasteurianus into E. coli by the methylase selection method...
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5614402 |
5' nucleases derived from thermostable DNA polymerase
A means cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is...
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5602019 |
DNA encoding mammalian phosphodiesterases
The present invention relates to novel purified and isolated nucleotide sequences encoding mammalian Ca 2 + /calmodulin stimulated phosphodiesterases (CaM-PDEs) and cyclic-GMP-stimulated...
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5589329 |
Methods and compositions for detecting base pair mismatches
The present invention discloses improved compositions and methods for detecting mutations, including single base changes, in nucleic acid sequences using RNase protection assays. The improvements...
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5550034 |
Apolipoprotein B mRNA editing protein compositions and methods
The present invention provides a protein that edits apo B RNA. A polynucleotide that comprises a DNA sequence that encodes an apo B RNA editing protein and an expression vector comprising such a...
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5547867 |
Fermentation processes for preparing soluble ricin A
Processes for preparing polypeptides such as ricin A such that soluble polypeptide may be obtained. The pH may be adjusted during the process or the temperature decreased during the terminal...
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5543308 |
Isolated DNA encoding the FSEI restriction endonuclease and related methods for producing the same
The present invention is directed to a method for cloning and producing the FseI restriction endonuclease by 1) introducing the restriction endonuclease gene from Frankia species into a host...
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5540925 |
Compositions comprising ONCONASE (TM) and STELAZINE (TM) or TAMOXIFEN (TM)
A pharmaceutical to be sold under the ONCONASE trademark, as described in pending commonly owned application application Ser. No. 07/436,141 filed Nov. 13, 1989 is combined with other drugs sold...
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5532153 |
Method for cloning and producing the SacI restriction endonuclease
The present invention relates to recombinant DNA which encodes the SacI restriction endonuclease and modification methylase, and production of SacI restriction endonuclease from the recombinant DNA.
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5529775 |
Compositions comprising ONCONASE(™) and cisplatin, melphalan, or doxorubicin HCl
A pharmaceutical known by the trademark ONCONASE, as described in pending commonly owned application application Ser. No. 07/436,141 filed Nov. 13, 1989, is combined with drugs sold under the names...
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5523232 |
Use of restriction endonucleases against viruses, including HIV
Restriction endonucleases are administered to a patient topically or internally to prevent or ameliorate viral infection. The endonucleases are also useful to treat products, such as blood and...
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RE35248 |
Method for producing the Hinc II restriction endonuclease and methylase
The present invention is directed to a method for cloning and producing the Hinc II restriction endonuclease by (1) introducing the restriction endonuclease gene from Haemophilus influenzae Rc into...
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5516678 |
Method for producing the SSPI restriction endonuclease and methylase
The present invention is directed to a method for cloning and producing the SspI restriction endonuclease by 1) introducing the restriction endonuclease gene from Sphaerotilus species into a host...
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5516634 |
Molecular basis of the human platelet bra/brb alloantigen system and applications thereof
Based on the discovery of the amino acid and nucleotide differences which distinguish the Br a and Br b allelic forms of the membrane glycoprotein GPIa, and which comprise the diallelic Br...
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5500370 |
Thermostable ribonuclease H and genetic constructs therefore
An essentially pure preparation of thermostable RNase H isolated from Thermus flavus is disclosed. In one preferable form of the invention, the RNase H is capable of biological activity after...
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5498535 |
Method for direct cloning of nuclease genes in E. coli
The present invention discloses a novel method for the direct cloning of nuclease genes such as restriction endonuclease genes in E. coli. In addition, there is provided a novel strain which...
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5496714 |
Modification of protein by use of a controllable interveining protein sequence
The present invention is directed to modified proteins and methods of their production. The modified proteins comprise a controllable intervening protein sequence (CIVPS) inserted into a target...
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5492823 |
Method for direct cloning and producing the BsoBI restriction endonuclease in E. coli
The present invention discloses a novel method for the direct cloning of nuclease genes such as restriction endonuclease genes in E. coli. In addition, there is provided a novel strain which...
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5491060 |
Mutant strain of E. coli for detection of methyltransferase clones
E. coli bacterial strains encoding a restriction gene that degrades methylated DNA, and prevents cloning of genes expressing the methyltransferase responsible for methylation, are mutated by a...
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5491080 |
Plants resistant against plural viruses and method for producing them using as RNase genes
This invention relates to a method for producing a plant having resistance against RNA viruses by integrating a DNA sequence, which encodes a protein having an enzyme activity specifically breaking...
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5489524 |
Chimeric protein that has a human Rho motif and deoxyribonuclease activity
The present invention relates, in general, to endo-exonucleases. In particular, the present invention relates to DNA segments encoding for a polypeptide having an amino acid sequence corresponding...
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5487994 |
Insertion and deletion mutants of FokI restriction endonuclease
The present invention reveals the construction of several insertion (4, 8, 12, 18, 19 or 23 amino acid residues) and deletion (4 or 7 amino acid residues) mutants of the linker region of FokI...
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5474921 |
Expression and purification of phosphoinositide-specific phospholipase C-γ
The present invention is directed to an assay which is used to determine the inhibitory activity of a test compound against a particular phosphoinositide-specific phospholipase C enzyme, that...
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5472872 |
Recombinant CviJI restriction endonuclease
DNA sequences encoding a novel restriction endonuclease (designated R.CνiJI) and variants thereof are disclosed along with method and materials for production of the same by recombinant methods. A...
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5459055 |
Thermostable ribonuclease H isolated from Thermus flavus
An essentially pure preparation of thermostable RNase H isolated from Thermus flavus is disclosed. In one preferable form of the invention, the RNase H is capable of biological activity after...
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5457044 |
Method for collection of aerosolized proteins by inert filtration
A method for collecting an aerosolized therapeutic polypeptide of interest, such as recombinant human deoxyribonuclease I (rhDNase), is provided which enables a determination as to the effect...
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5451519 |
Cloning restriction endonuclease genes by modulating methyltransferase activity
The present invention relates to a method for cloning genes that encode restriction endonucleases by altering the level of a methyl donor co-factor of a DNA methyltransferase that protects the DNA...
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5441881 |
NSP7524V restriction-modification genes
To provide Nsp7524V restriction-modification genes and a method for producing Nsp7524V restriction enzyme and Nsp7524V modification enzyme by using a novel microorganism having, introduced...
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5439815 |
Restrictocin-like ribotoxin analogues comprising only one cysteine available for covalent linkage to a partner
Ribotoxins, such as restrictocin, mitogillin and alpha-sarcin which do not contain a cysteine for linkage to antibodies for immunotoxin production need to be modified for such linkage without...
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5436150 |
Functional domains in flavobacterium okeanokoities (foki) restriction endonuclease
The present inventors have identified the recognition and cleavage domains of the FokI restriction endonuclease. Accordingly, the present invention relates to DNA segments encoding the recognition...
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5434068 |
Method for cloning and producing the BglII restriction endonuclease and modification methylase
The present invention is directed to a method for cloning and producing the BglII endonuclease by: 1) introducing the restriction endonuclease gene from Bacillus globigii into a host whereby the...
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5434058 |
Apolipoprotein B MRNA editing protein compositions and methods
The present invention provides a protein that edits apo B RNA. A polynucleotide that comprises a DNA sequence that encodes an apo B RNA editing protein and an expression vector comprising such a...
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5420032 |
Homing endonuclease which originates from chlamydomonas eugametos and recognizes and cleaves a 15, 17 or 19 degenerate double stranded nucleotide sequence
The present invention relates to a homing endonuclease which originates from Chlamydomonas eugametos, and was overproduced in E. coli, purified and characterized. The homing endonuclease of the...
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5418150 |
Method of cleaving DNA
A method of cleaving substrate DNA with a restriction enzyme, wherein the substrate DNA is resistant to cleavage by the restriction enzyme, is disclosed. The method comprises co-incubating the...
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5405760 |
Process for producing recombinant McrBC endonuclease and cleavage of methylated DNA
The present invention relates to a recombinant McrBC endonuclease obtainable from Escherichia coli, two components of which, McrB L and McrC, have been purified in active form. McrBC is active in...
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5405768 |
Method for cloning and producing the AATII and ALUI restriction endonuclease and methylase and related method for overexpressing restriction endonucleases
The present invention provides a novel approach to the production of restriction endonucleases. More specifically, there is provided a novel method for the overexpression of these enzymes, which...
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5391487 |
Restriction endonuclease SgfI from Streptomyces griseoruber
A restriction endonuclease is provided. The enzyme, designated SgfI, recognizes the DNA sequence ##STR1## and cleaves the DNA sequence at a position indicated by the arrows and is preferably...
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5389537 |
Nuclease having altered specificity
A ribonuclease molecule altered at a single amino acid, relative to its wild-type form, displays altered substrate specificity and substrate binding mechanism. The altered protein cleaves RNA...
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