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6403354 |
Method for cloning and expression of BstYI restriction endonuclease and BstYI methylase in E. coli and purification of BstYI and M.BstYI enzymes
The present invention relates to recombinant DNA which encodes the BstYI restriction endonuclease as well as BstYI methyltransferase, expression of BstYI restriction endonuclease and M.BstYI in E....
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6395963 |
Nematode-inducible regulatory DNA sequences
Nematode-inducible promoter sequences are disclosed. These sequences can be used for expressing DNA sequences in plant cells. Chimeric DNA constructs comprising the nematode-inducible promoter...
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6395531 |
Method for cloning and expression of MlyI restriction endonuclease and MlyI methylase and BstNBII methylase in E. coli
The present invention relates to recombinant DNA which encodes the MlyI restriction endonuclease as well as the MlyI and BstNBII methyltransferases and expression of MlyI restriction endonuclease...
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6391608 |
Method for cloning and expression of PleI restriction endonuclease and PleI and BstNBII methylases in E. coli
The present invention relates to recombinant DNA which encodes the PleI restriction endonuclease as well as PleI and BstNBII methyltransferase and expression of PleI restriction endonuclease and...
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6387681 |
Method for cloning and expression of NHEI restriction endonuclease in E. coli.
The present invention relates to recombinant DNA which encodes the NheI restriction endonuclease as well as NheI methyltransferase, expression of NheI restriction endonuclease from E. coli cells...
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6365374 |
2′-deoxy-2′-alkylnucleotide containing nucleic acid
2′-deoxy-2′-alkylnucleotides useful for stabilizing enzymatic nucleic acid molecules and antisense molecules.
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6365379 |
Zinc finger peptide cleavage of nucleic acids
Selective cleavage of single stranded nucleic acids can be effected by contacting the nucleic acid with a zinc finger peptide in dimeric form. Dimerization results from diminution or elimination of...
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6348343 |
Human DNase I variants
The present invention relates to amino acid sequence variants of human DNase I that have reduced binding affinity for actin. The invention provides nucleic acid sequences encoding such...
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6335190 |
Method for cloning and producing the BsmI restriction endonuclease in E. coli
The present invention relates to recombinant DNA which encodes the BsmI restriction endonuclease as well as BsmI methyltransferases, expression of BsmI restriction endonuclease in E. coil cells...
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6333159 |
Cold sensitive mutant DNA polymerases and methods of use thereof
Provided are mutant DNA polymerases having at least one mutation which exhibit substantially reduced polymerase activity at 25° C. when compared to the same DNA polymerases without the at least...
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6326199 |
Gapped 2' modified oligonucleotides
Oligonucleotides and other macromolecules are provided that have increased nuclease resistance, substituent groups for increasing binding affinity to complementary strand, and sub-sequences of...
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6316202 |
Nucleic acids encoding cold sensitive mutant DNA polymerases
Provided are mutant DNA polymerase having at least one mutation which exhibit substantially reduced polymerase activity at 25° C. When compared to the same DNA polymerase without the at least one...
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6300109 |
Plasmid-derived llad II restriction-modification system from lactococcus lactis
A number of type II restriction-modification (R-M) systems have been derived from plasmids found in Lactococcus lactis strains from the Danish starter culture TK5. The R-M systems LlaAI, LlaBI and...
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6291741 |
Method for the production of modified plants
Methods for the production of modified plants are provided. In these methods a first line and a second line are crossed to produce a plant having a phenotypic trait, wherein neither the first or...
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6284468 |
Composition and methods for determining the activity of DNA-binding proteins and of initiation of trascription
This invention provides plasmids that are useful in detecting and determining the DNA-binding activity of sequence-specific DNA-binding molecules. The invention further provides plasmids that are...
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6284463 |
Method for detection of mutations
A method and a kit for detecting a mutation from a non-mutated sequence of a target polynucleotide in a sample, by using a mismatch binding protein. The method comprises: a) providing non-mutated...
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6280992 |
Type II restriction endonuclease, Hpy99I, obtainable from Helicobacter pylori J99 and a process for producing the same
In accordance with the present invention, there is provided a novel restriction endonuclease and its DNA obtainable from Helicobacter pylori J99 (NEB#1237), hereinafter referred to as "Hpy99I",...
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6280991 |
Engineered cytotoxic ribonclease
Modified ribonucleases belonging to the RNase A superfamily of ribonucleases is disclosed. Each modified ribonuclease has a steric hindrance moiety added to it in the loop region corresponding to...
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6271369 |
Chimeric molecules targeted to viral RNAs
Chimeric molecules comprising a virus targeting antisense oligonucleotide moiety attached to an activator of 2-5A-dependent RNase.
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6265196 |
Methods for inactivating target DNA and for detecting conformational change in a nucleic acid
The present invention reveals a method for enzymatically inactivating a target DNA, a method for detecting conformational change in a nucleic acid, and a method for detecting the presence of a...
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6262344 |
Nematode-inducible plant gene promoter
An isolated DNA fragment that promotes root knot or cyst nematode indcuible transcription of a coding sequence downstream of and operably linked to the fragment in at least an Arabidopsis thaliana...
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6258583 |
Type II restriction endonuclease, Hpy188I, obtainable from helicobacter pylori J188 and a process for producing the same
In accordance with the present invention, there is provided a novel restriction endonuclease and its DNA obtainable from Helicobacter pylori J188 (NEB#1174), hereinafter referred to as "Hpy188I",...
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6255081 |
Thermostable flap endonuclease derived from hyperthermophile bacterium belonging to the genus pyrococcus
The present invention relates to a thermostable Flap endonuclease whose optimum temperature is 75° C. or more and DNA coding for (a) a protein consisting of the amino acid sequence shown in SEQ ID...
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6252138 |
Pathogen-induced plant promoters
New pathogen-induced promoters are provided, particularly nematode-induced promoters, which are characterized by their selective induction of expression in the vicinity of the pathogen infection...
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6251648 |
Gene encoding human Dnase
A human DNase polypeptide and DNA (RNA) encoding such polypeptide and a procedure for producing such polypeptide by recombinant techniques is disclosed. Also disclosed are methods for utilizing...
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6245545 |
Method for cloning and producing the SwaI restriction endonuclease
The present invention relates to the recombinant DNA which encodes the SwaI restriction endonuclease, modification methylase, and the production of SwaI restriction endonuclease from the...
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6239257 |
Family of proteins belonging to the pancreatic ribonuclease a superfamily
A protein family includes four proteins that are bioactive against human tumor cell lines. The proteins are derived from eggs of the Rana pipiens frog, and are members of the superfamily of...
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6238904 |
Type II restriction endonuclease, HpyCH4III, obtainable from Helicobacter pylori CH4 and a process for producing the same
In accordance with the present invention, there is provided a novel restriction endonuclease and its DNA obtainable from Helicobacter pylori CH4 (NEB#1236), hereinafter referred to as "HpyCH4III",...
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6238901 |
Type II restriction endonuclease, HPY 188 III, obtainable from Helicobacter pylori J188 and a process for producing the same
In accordance with the present invention, there is provided a novel restriction endonuclease and its DNA obtainable from Helicobacter pylori J188 (NEB#1174), hereinafter referred to as "Hpy188III",...
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6210945 |
Method for cloning and producing the RsaI restriction endonuclease in E. coli and purification of the recombinant RsaI restriction endonuclease
RsaI, a restriction enzyme from the bacterium Rhodopseudomonas sphaeroides, recognizes the DNA sequence 5'-GTAC-3'. Because RsaI is commercially valuable, we sought to overproduce it by cloning the...
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6194188 |
Type II restriction endonuclease, HpyCH4IV, obtainable from Helicobacter pylori CH4 and a process for producing the same
In accordance with the present invention, there is provided a novel restriction endonuclease and its DNA obtainable from Helicobacter pylori CH4 (NEB#1236), hereinafter referred to as "HpyCH4IV",...
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6187589 |
Vectorial cloning system of DNA
The invention relates to a vectorial cloning system consisting of a sequence of nucleotides, containing a fusion sequence and one or several restriction endonucleases, recognition sites for...
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6147282 |
Method of controlling the fertility of a plant
The present invention is drawn to a method of controlling gene expression in plants. Specifically, the method comprises obtaining a transgenic plant comprising at least two receptor expression...
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6133009 |
Type II Restriction endonuclease, HpyCH4V, obtainable from helicobacter pylori CH4 and a process for producing the same
In accordance with the present invention, there is provided a novel restriction endonuclease and its DNA obtainable from Helicobacter pylori CH4 (NEB#1236), hereinafter referred to as "HpyCH4V",...
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6133008 |
Method for cloning and producing the TfiI Restriction endonuclease in E. coli
A genomic DNA library of Thermus filiformis was constructed using pBR322 as a cloning vector. The methylase selection method was used to clone the TfiI methylase gene (tfiIM). A clone carrying an...
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6130078 |
Method for cloning the NSPHI restriction-modification system in E. coli and producing the recombinant NSPHI restriction endonuclease
The present invention relates to recombinant DNA molecules encoding NspHI restriction endonuclease and methylase and to method to use premodified E. coli K strain RR1 (λDE3) for overexpression of...
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6110462 |
Enzymatic DNA molecules that contain modified nucleotides
The present invention discloses catalytic or enzymatic DNA molecules that contain a modified nucleotide and that are capable of cleaving nucleic acid sequences or molecules, particularly RNA, in a...
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6090606 |
Cleavage agents
The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to...
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6074876 |
Genetic transformation using a PARP inhibitor
The invention concerns a process for producing transgenic plant cells, which comprises: contacting a culture of plant cells with an inhibitor of poly-(ADP-ribose) polymerase, prior to...
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6071734 |
HBV polymerase, RNase H enzyme derived from HBV polymerase, processes for preparation and uses for screening antiviral agents thereof
The present invention relates to hepatitis B virus (hereinafter it refers to HBV) polymerase containing a histidine tag, RNase H enzyme derived from HBV polymerase and processes for preparation...
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6066487 |
Method for cloning and expression of BsrFI restriction endonuclease in E. coli
BsrFI restriction enzyme was purified from Bacillus stearothermophilus to near homogeneity. The protein was sequenced to obtain its N-terminus amino acid sequence. A set of denegerate primers were...
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6060302 |
Human phospholipase C-α and DNA sequence encoding the same
Genes encoding a human PLC-α polypeptide are provided. An expression vector containing these genes and a transformant having the expression vector are provided. The human PLC-α polypeptide can be...
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6054307 |
Octanucleotide restriction endonuclease, Srf I, and method for producing same
This invention provides restriction endonuclease Srf I, which is capable of recognizing the eight nucleotide base palindromic sequence shown below on a double stranded DNA molecule and cleaving the...
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6048719 |
Method for cloning and producing the DraIII restriction endonuclease
The present invention relates to the recombinant DNA which encodes the DraIII restriction endonuclease modification methylase, and the production of DraIII restriction endonuclease from the...
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6048731 |
Method for cloning and producing the SgrAI restriction endonuclease
The present invention relates to the recombinant DNA which encodes the SgrAI restriction endonuclease and the MspI modification methylase, and the production of SgrAI restriction endonuclease from...
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6045793 |
Recombinant ribonuclease proteins
The invention relates to ribonucleases derived from a native ribonuclease found in the oocytes of Rana pipiens. Various humanized and recombinant forms of these molecules are described as well as...
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6037152 |
Method for reducing carryover contamination in an amplification procedure
The present invention provides an efficient and economical method for reducing carryover contamination in an amplification procedure. The method of the present invention enables background caused...
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6037162 |
DNA segments encoding a domain of HO-endonuclease
The invention provides an isolated DNA segment encoding a domain of HO-endonuclease, said segment being selected from the group consisting of an isolated DNA segment encoding the N-terminus of...
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6027929 |
Method for cloning and producing the NspI restriction endonuclease in E. coli and purification of the recombinant NspI restriction endonuclease
The present invention relates to clones which express the recombinant NspI restriction endonuclease using a NspI methylase premodified E. coli K strain RR1 (λDE3) and to methods for producing and...
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6025179 |
Method for cloning and producing the SnaBI restriction endonuclease and purification of the recombinant SnaBI restriction endonuclease
The methylase selection method was used to clone the SnaBI methylase gene (snaBIM) from Sphaerotilus natans (ATCC 15291). An active SnaBI methylase was cloned in E. coli using pSnaBI-2, a pUC19...
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