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5206156 Process for the preparation of a particulate antimicrobial product, antimicrobial product obtained and applications thereof  
The invention relates to a process for the preparation of a particulate antimicrobial product from the LP system of the lactoperoxidase enzyme/oxygen donor/oxidizable substrate (if needed). The...
5206135 Use of the oxygenase activity of acetolactate synthase for herbicide detection  
A process for the detection of herbicides that inhibit acetolactate synthase (EC 4.1.3.18) by use of the enzyme, a suitable substrate (pyruvate, α-aceto-lactate, α-ketobutyrate, or...
5206148 Method for assaying aliphatic alcohol, aliphatic aldehyde or ω-carboxylic acid derivatives thereof  
A method for assaying aliphatic alcohol, aliphatic aldehyde or ω-carboxylic acid derivatives thereof, using a novel ω-carboxyalcohol oxidase which catalyzes at least one of the following...
5204261 Catalase-negative Pichia pastoris  
A novel strain of P. pastoris and an alcohol oxidase free of catalase activity produced therefrom is provided.
5200335 Phenylethanol dehydrogenase capable of reducing acetophenone to R(+)-phenylethanol  
A phenylethanol dehydrogenase capable of catalyzing the reduction of acetophenone to R(+)-phenylethanol in the presence of NADPH was isolated from Lactobacilli such as Lactobacillus kefir. The...
5196317 Overexpression of proteins in recombinant host cells  
The invention relates to a process for achieving high, thermally-induced expression rates, which last for a relatively long period of time, of proteins in recombinant cells, preferably in E. coli...
5187088 Choline oxidase and method for producing the same  
A novel choline oxidase being thermostable at 50° C. at pH 7 to 9 is disclosed. A method for producing the enzyme by using actinomycetes is also disclosed.
5180672 Ascorbate oxidase isolated from Acremonium sp. HI-25 ferm BP-3124  
An ascorbate oxidase having an optimum pH range of 3.5-4.5 is isolated from Acremonium sp. Hi-25 BP-3124. The ascorbate oxidase is used in foods or drinks to oxidize ascorbate to prevent damage to...
5173416 L-carnitine dehydrogenase from alcalgenes  
A new class of L-carnitine dehydrogenase is disclosed, which is stable in solution and has a residual activity of greater than 70% after one week in a pH 9.0 buffer solution at 5° C., a molecular...
5171681 Omega-carboxyalcohol oxidase  
A novel ω-carboxyalcohol oxidase catalyzes at least one of the following reactions: a) R--CH 2 OH+O 2 ➝R--CHO+H 2 O 2 b) R--CHO+O 2 +H 2 O➝R--COOH+H 2 O 2 wherein R is alkyl,...
5164314 Microbiologically-prepared diacetyl reductase  
A process for the enzymatic preparation of acetoin, particularly (+)-acetoin, employs a diacetyl reductase which can be obtained from a yeast or Lactobacillus strain.
5162516 Cloning and sequencing of the alcohol dehydrogenase II gene from Zymomonas mobilis  
The alcohol dehydrogenase II gene from Zymomonas mobilis has been cloned and sequenced. This gene can be expressed at high levels in other organisms to produce acetaldehyde or to convert...
5162203 Methods of measuring isozymes and isozyme classes of alcohol dehydrogenase  
The present invention provides fluorescence-based methods for sensitively detecting total ADH activity in human sera and selectively measuring the activity of different classes of ADH in human sera...
5156966 L-carnitine dehydrogenase and process for its production  
An isolated L-carnitine dehydrogenase is disclosed, which is stable in solution and has a residual activity of greater than 70% after one week in a pH 9.0 buffer solution at 5° C. Also disclosed...
5149646 Process for isolating galactose oxidase  
A method of isolating and purifying galactose oxidase from a fungus media is disclosed. The supernatant liquid from the fermentation broth is subjected to ultrafiltration and then separated on a...
5147793 Biocatalytic oxidation using soybean peroxidases  
Biocatalytic oxidative processes wherein oxidizable substrates are reacted with peroxides in the presence of a peroxidase such as soybean peroxidase or another legume peroxidase; an oxidative...
5143828 Method for synthesizing an enzyme-catalyzed polymerized monolayer  
A method for synthesizing enzyme-catalyzed polymers using the Langmuir-Blodgett technique. In one embodiment, the process comprises spreading one or more enzyme-polymerizable monomers on a...
5141854 Enzymatic composition for ethanol assay  
An enzymatic assay composition for ethanol determination is provided. A compound of the formula H 2 N--(CH 2 ) n --CHR--CH 2 --NH 2 where R is H, CH 3 , or OH and N is 0-4 acts as a...
5137821 Gene and process of making glucose-6-phosphate dehydrogenase  
Glucose-6-phosphate dehydrogenase which is an enzyme for assaying creatinekinase for diagnolyzation of myocardial infraction is produced by culturing a transformant having DNA which codes amino...
5135860 Process for preparing glyoxylic acid/aminomethylphosphonic acid mixtures  
The invention provides a process for producing mixtures of glyoxylic acid and aminomethylphosphonic acid. The process comprises reacting glycolic acid and oxygen in an aqueous solution in the...
5134071 Polymerization and copolymerization of proteins  
A method for producing a polyprotein having at least 10 units, and often as many as 50 to 100 and more units held together by sulfur to sulfur or sulfur to carbon bonds is disclosed. Each unit...
5124141 Method for diagnosing malaria  
A diagnostic method for the detection of human malaria infections. Specifically, blood samples are combined with a reagent containing 3-acetyl pyridine adenine dinucleotide (APAD), a substrate...
5118621 Non-steroidal suicide substrates of hydroxysteroid dehydrogenase  
Compounds, such as 1-(4'-nitrophenyl)-2-propen-1-ol, are disclosed which are highly selective non-steroidal suicide substrates for rat liver 3α-hydroxysteroid dehydrogenase.
5116750 Selectable fusion protein having aminoglycoside phosphotransferase activity  
A novel a selectable fusion protein having aminoglycoside phosphotransferase activity is disclosed. The marker comprises the coding sequences for aminoglycoside phosphotransferase I (APH-I) which...
5114853 Recombinant DNA, transformant containing said DNA, and process for preparing heat-stable glucose dehydrogenase by use of said transformant  
A recombinant DNA replicable and expressible in Escherichia coli is disclosed which encodes glucose dehydrogenase originating from Bacillus megaterium. A DNA sequence encoding the glucose...
5104797 Process for preparing 5-C-hydroxymethyl aldohexose-based compounds  
Processes of the present invention provide economical methods for preparing derivatives 5-C-hydroxymethyl-D-aldohexose and their bicyclic anhydro tautomeric forms. The 5-C-hydroxymethylation...
5098836 Deoxygenation in field preparation of polymers in aqueous solution  
In a process for polymerizing monomers with free-radical initiators in aqueous solution to produce polymers useful in oil field applications, wherein said polymerization process employs an...
5096820 Process for manufacturing xylitol and xylitol-rich products  
Process for manufacturing xylitol and xylitol-rich products, characterized in that it consists of: enzymatically isomerizing at M 1 D-xylulose syrup into syrup containing D-xylose and...
5094943 Process and reagent for the improved quantitative colorimetic determination of hydrogen peroxide  
The present invention provides a process for the improved colorimetric determination of hydrogen peroxide as formed by a hydrogen peroxide-producing oxidase, by addition of a chromogenic system and...
5094951 Production of glucose oxidase in recombinant systems  
The present invention provides recombinant polynucleotides which encode glucose oxidase (GO). It also provides recombinant expression systems which produce, and when desired, secrete active GO and...
5093254 Aqueous two-phase protein extraction  
An aqueous two-phase protein partitioning system is disclosed which employs polyvinylpyrrolidone as the upper phase and maltodextrin as the lower phase and provides a low-cost system for protein...
5089388 Antibodies for salicylate and their preparation  
Conjugates of salicylic acid and certain poly(amino acids), which are either antigenic or enzymes, are provided. Antibodies raised against the antigenic poly(amino acids) and the enzyme conjugates...
5082770 Method for quantitative determination of polyamines  
A method for the quantitative determination of polyamines, which comprises allowing a polyamine oxidizing enzyme, an ω-aminoalkylaldehyde dehydrogenase, an oxidized nicotinamide coenzyme and, as...
5077206 Process for preparing L-rhamnose  
The invention provides a process for preparing L-rhamnose by hydrolyzing a rhamnosidic bond of a glycoside having rhamnose in a terminal position, by enzymatically hydrolyzing the glucoside with an...
5068190 N-acetylhexosamine-dehydrogenase, process for producing same, method for the quantitative analysis of N-acetylglucosamine or N-acetylgalactosamine using same and kit for use in the quantitative analysis  
N-Acetylhexosamine-dehydrogenase which takes off hydrogen from N-acetylglucosamine or N-acetylgalactosamine to convert them to N-acetylglucosaminolactone or N-acetylgalactosaminolactone,...
5034320 Vinblastine synthesis  
The reaction of 3',4'-anhydrovinblastine with a Catharanthus roseus-derived protein fraction to form vinblastine is improved by conducting the reaction in the presence of a reducing agent such as...
5028539 Ethanol production using engineered mutant E. coli  
The subject invention concerns novel means and materials for producing ethanol as a fermentation product. Mutant E. coli are transformed with a gene coding for pyruvate decarboxylase activity. The...
5026642 Process for the enzymatic production of ketones  
In a process for the reduction of ketones, the keto compounds are enantioselectively reduced to give secondary alcohols by means of microorganisms. Keto compounds of the formula R 3 NH--CHR 1 ...
5026648 Microbial culture having catechol 1,2-oxygenase activity  
This invention provides novel strains of microorganisms (e.g., Pseudomonas putida Biotype A) which are capable of converting substrates such as toluene or catechol to muconic acid quantitatively by...
5019505 Enzymes having alpha-glycerol-3-phosphate oxidase activity, production and use thereof  
An enzyme having α-glycerol-3-phosphate oxidase activity characterized in that it has a Km of less than 2.5 mM is disclosed, as is the production and use thereof. More particularly, such enzymes...
5019496 Photopolymerization diagnostic test composition and method for immunoassay and nucleic acid assay  
A diagnostic test composition for detecting and measuring an analyte possessing biologic activity comprising (a) a photocatalyst system capable of converting a monomer to a polymer upon exposure...
5011775 Enzymatic process for the preparation of rosmarinic acid  
A process is disclosed for the production of rosmarinic acid of the formula ##STR1## by enzymatic reaction of 3-(3,4-dihydroxyphenyl)-lactic acid with caffeoyl-coenzyme A in the presence of...
5010007 Composition for removing oxygen in foodstuff and drinks  
The present invention relates to a composition for removing both molecular and free-radical oxygen from foodstuff materials capable of being degraded by oxidation which comprises the incorporation...
5010005 Bio-oxidation of high alcohols in non-aqueous reaction media  
Whole cells of methylotrophic yeasts are able to oxidize benzyl alcohol to benzaldehyde in aqueous reaction media. However, the low water solubility of the reactant and product of this...
5008193 Ascorbic acid intermediates and process enzymes  
Methods for producing ascorbic acid using recombinant means comprising the transfer of genetic material by conjugation, a host cell lacking, entirely or to such an extent as not to be commercially...
5004690 Ascorbic acid intermediates and process enzymes  
A process for the preparation of 2-keto-L-gulonic acid (2-KLG) from 2,5-diketo-D-gluconic acid (2,5-DKG) using a DNA sequence encoding 2,5-DKG reductase. The 2-KLG is a precursor for the synthesis...
5002886 Oxidoreductase and the preparation thereof  
An oxidoreductase is provided which, in the presence of nicotinamide adenine dinucleotide (NAD + ) as a co-enzyme, will catalyse the oxidation of polyols having at least one hydroxyl group on each...
4956290 Large scale process for the purification of alcohol oxidase  
A process for the purification of alcohol oxidase from whole cells of Pichia pastoris grown on methanol by the sequential steps of autolysis, crossflow filtration, ultrafiltration and...
4950602 Inhibition of lactate production by pyruvate adducts  
The present invention relates to inhibitors of lactate dehydrogenase that can cross the blood brain barrier and there is described herein the preparation of several analogues of...
4927753 Quantitative determination of L-fucose  
A method is provided for the quantitative determination of L-fucose in a sample solution. According to the method an L-fucose dehydrogenase having its optimum pH around neutrality is allowed to act...