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5487978 |
Method, composition and device for the determination of cholesterol using cholesterol oxidase obtained from bacterial strain NRRL B-18713
The present invention provides a kinetic or rate method for the determination of cholesterol in free or bound form with of a cholesterol oxidase enzyme obtained from Bacterial strain NRRL B-18713...
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5472862 |
Sorbitol oxidase from Xanthomonas maltophilia FERM BP-4512
An isolated sorbitol oxidase from Xanthomonas maltophilia FERM BP-4512 is disclosed. The oxidase catalyzes the reaction of D-sorbitol+O 2 ➝D-glucose+H 2 O 2 , has a substrate specificity for...
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5464768 |
Enhanced nitrite production in transfected murine cells
Mamammalian cell lines capable of enhanced nitrite production are by transfecting a murine macrophage or murine thymoma with barley nitrate reductase gene (NR).
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5451402 |
Method for killing yeast and sporular microorganisms with haloperoxidase-containing compositions
Methods and compositions are provided for killing or inhibiting the growth of yeast or sporular microorganisms by contacting the microorganisms, in the presence of a peroxide and chloride or...
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5451517 |
D-arabinitol dehydrogenase from Candida tropicalis ATCC 750 or Candida shehatae
A D-arabinitol dehydrogenase enzyme is disclosed. The enzyme is capable of catalyzing the oxidation of D-arabinitol and substantially incapable of catalyzing the oxidation of D-mannitol and is...
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5439813 |
Production of glyoxylic acid with glycolate oxidase and catalase immobilized on oxirane acrylic beads
A process for the production of glyoxylic acid by reacting glycolic acid with oxygen in an aqueous solution in the presence of an amine buffer capable of forming a chemical adduct with glyoxylic...
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5439943 |
Non-steroid and non-prostanoid inhibitors of steroid and prostaglandin transforming enzymes
Compounds such as 1-(4'-nitrophenyl)-2-propen-1-ol are disclosed which are non-steroidal mechanism-based inactivators of rat liver 3α-hydroxysteroid dehydrogenase. The corresponding ketones are...
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5437989 |
Alcohol/aldehyde dehydrogenase from Gluconobacter oxydans DSM 4025 FERM BP-3812
A homogeneous alcohol/aldehyde dehydrogenase isolated from Gluconobacter oxydans DSM 4025 FERM BP-3812 is disclosed. The enzyme is capable in the presence of an electron acceptor, of catalyzing the...
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5432071 |
Variant E1 protein gene for pyruvate dehydrogenase complex and variant E1 protein of pyruvate dehydrogenase complex
The present invention relates to variant E 1 protein of pyruvate dehydrogenase complex of high activity in which arginine at the 146-position is replaced by proline in the amino acid sequence of...
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5424204 |
Method for stabilizing glucose 6-phosphate dehydrogenase with hydroxylamines, aldehyde scavengers, dimethylthiocarbamoyl chloride or 2-(2-aminoethylamino) ethanol
Glucose 6-phosphate dehydrogenase is stabilized in a clinical assay reagent with at least one stabilizer selected from among hydroxylamines, aldehyde scavengers dimethylthiocarbamoyl chloride,...
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5424196 |
Enzymatic process for preparing 7-aminocephalosporanic acid and derivatives
Transformation of Cephalosporin C or its derivatives and salts into 7-aminocephalosporanic acid or its derivatives by an enzymatic two stage process with enzymes immobilized on a solid matrix.
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5420008 |
Assay method and assay reagent for serum iron or unsaturated iron binding capacity
The present invention measures serum iron by liberating iron from transferrin under acid conditions, and contacting a test sample with a reaction system which utilizes at least aconitase, a...
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5411879 |
Fatty acyl reductases
By this invention, a solubilized seed-plant fatty acyl reductase protein is provided, wherein said protein is active in the formation of a fatty alcohol from a fatty acyl substrate. Of special...
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5403731 |
Process of producing modified superoxide dismutase
A modified superoxide dismutase represented by the formula: ##STR1## (wherein R is as defined below; SOD is a residue of superoxide dismutase) is produced, in a shortened period of reaction with a...
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5401649 |
2-methyl-4-hexene-and 3-methyl-5-heptene-1,2-diol derivatives
2-methyl-4-hexene- and 3-methyl-5-heptene-l,2-diol derivatives are disclosed together with methods for preparing such derivatives. Where the derivative is a 2-methyl-4-nexene- or...
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5389369 |
Halo peroxidase containing compositions for killing yeast and sporular microorganisms
Methods and compositions are provided for killing or inhibiting the growth of yeast or sporular microorganisms by contacting the microorganisms, in the presence of a peroxide and chloride or...
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5387109 |
Fructosylamine deglycase and a method of producing it
Disclosed are a novel fructosylamine deglycase characterized by the specificity to amadori compounds of catalyzing oxidation of the compounds to produce an α-ketoaldehyde, an amine derivative and...
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5385828 |
Method for determining the relative amounts of all cholesterol-containing lipoproteins in body fluids
The invention refers to a method for determining the relative amounts of all cholesterol-containing lipoproteins in body fluids comprising electrophoretically separating the lipoproteins of an...
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5356794 |
Purified enzymatic preparation derived from chromobacterium violaceum, method for preparing it and its use in the production of α, β-dehydrotryptophanyl-peptides
Purified enzymatic preparation derived from Chromobacterium violaceum, having a dehydrogenase activity, method for preparing it and its use in the production of α,β-dehydrotryptophanyl-peptides....
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5354676 |
Recombinant modified thermostable NAD-dependent dehydrogenases and process for their production
A recombinant thermophilic NAD-dependent dehydrogenase having a hydrophobic amino acid at position 104 and/or 102. The enzymes are effective in catalysing the dehydrogenation of homologues of...
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5352599 |
Co-enzyme-independent L-sorbosone dehydrogenase of gluconobacter oxydans: isolation, characterization, and cloning and autologus expression of the gene
A novel coenzyme independent L-sorbosone dehydrogenase originating from a microorganism belonging to the genus Gluconobacter oxydans which acts on L-sorbosone to produce 2-keto-L-gulonic acid. The...
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5344777 |
Structural gene of membrane-bound alcohol dehydrogenase complex, plasmid containing the same and transformed acetic acid bacteria
There is provided a structural gene of membrane-bound alcohol dehydrogenase complex having a molecular size of about 7.0 kilo base which is derived from a microorganism belonging to the genus...
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5342767 |
Lactobacillus kefir alcohol dehydrogenase
The present invention discloses the preparation of R-configured trialkylsilylethynyl α-alcohols from similarly substituted α-ketones, and vice versa, catalyzed by the alcohol dehydrogenase of...
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5334508 |
Process and agent for the colorimetric determination of an analyte by means of enzymatic oxidation
The present invention provides a process for the colorimetric determination of an analyte by means of enzymatic oxidation of the analyte in the presence of an electron acceptor and determination of...
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5316917 |
Automated analysis of free l-carnitine and total short-chain acylcarnitine
Disclosed is a method of assaying free L-carnitine and total short-chain acylcarnitine in blood. The method uses essentially protein-free samples of plasma or serum and comprises (a) obtaining a...
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5308770 |
Cloning and overexpression of glucose-6-phosphate dehydrogenase from Leuconostoc dextranicus
The invention concerns a glucose-6-phosphate dehydrogenase which contains the amino acid sequence shown in SEQ ID NO:1 as well as a DNA coding for it and a process for the isolation of an enzyme...
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5302520 |
Enzymatic synthesis of isotopically labeled carbohydrates
The present invention relates to a general method of enzymatic synthesis of isotopically labeled carbohydrates, sugars and nucleosides. Labeled citric acid cycle intermediates, amino acids and...
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5300436 |
Genetically modified tyrosine hydroxylase and uses thereof
Modification of the DNA encoding the enzyme tyrosine hydroxylase (TH) resulting in amino acid substitution in one of the first fifty five N-terminal residues, in particular replacing Ser-40 with...
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5298414 |
Detection of morphine using morphine dehydrogenase
Two enzymes, one an acetylmorphine carboxyesterase (AMCE), the other a morphine dehydrogenase (MDH), have been isolated from bacteria. The AMCE degrades heroin to morphine and the MDH oxidises...
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5298411 |
Glucose dehydrogenase from pseudomonas
A substantially purified glucose dehydrogenase is disclosed. The enzyme has activity at a temperature of from about 30° C. to about 65° C. at a pH of from about pH 6 to about pH 10 and has an...
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5296358 |
Process for the enzymatic preparation of cephalosporanic derivatives using a D-amino acid oxidase from Rhodotorula glutinis NCIMB 40412
The enzymatic preparation of cephalosporanic derivatives, or their salts, ving the formula: ##STR1## wherein R is --CO--COOH or --COOH, R 1 is H, OH, or --O--CO--R" and R" is an alkyl group with 1...
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5290926 |
Isolated DNA Encoding plant histidinol dehydrogenase
The present invention comprises cDNA coding for histidinol dehydrogenase from plants, the final step in histidine biosynthesis. The invention also comprises a novel method of purifying histidinol...
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5290683 |
Rapid analysis of ethanol in body fluids
An assay for directly and rapidly determining the alcohol concentration of a lower alcohol in a biological fluid without dilution, comprises a solid carrier. The carrier is impregnated with a...
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5288746 |
Liquid laundry detergents containing stabilized glucose/glucose oxidase as H.sub.2 O.sub.2 generation system
Liquid laundry detergent compositions containing glucose and glucose oxidase for generation of hydrogen peroxide during the laundering process are stabilized against premature hydrogen peroxide...
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5272077 |
Method for preparing a covalent conjugate of an oligonucleotide and an enzyme
A method is provided for preparing a covalent conjugate of an oligonucleotide and an enzyme, such as peroxidase. This conjugate can be used as a probe in hybridization assays and in polymerase...
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5270194 |
Stabilized glucose oxidase from Aspergillus Niger
An aqueous liquid composition is disclosed. The composition comprises a stabilized glucose oxidase solution having a pH of from about 6.0 to 8.0 and contains phosphate at a concentration of 1500 to...
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5268288 |
Mannitol oxidoreductase isolated from vascular plants
A mannitol oxidoreductase is isolated from vascular plants which is NAD + dependent and which converts mannitol to mannose. A preferred source of the mannitol oxidoreductase is Apium graveolens,...
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5266487 |
Apparatus for the treatment of lignocellulosic materials
An apparatus is disclosed for treating lignocellulosic materials, comprising an enzymatic conversion zone adapted to enzymatically convert alcohol to aldehyde and hydrogen peroxide, a...
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5260197 |
L-fucose dehydrogenase, a process for production thereof, quantitative assay for L-fucose using said enzyme and a kit for quantitative assay
L-Fucose dehydrogenase having the following Physicochemical properties (1) and (2): (1) Action and substrate specificity It withdraws hydrogen from L-fucose and converts into L-fuconolactone...
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5258286 |
Method of terminating isocitrate dehydrogenase reaction
Urea, creatinine, creatine, triglycerides, or the like in a specimen can be accurately determined by terminating an isocitrate dehydrogenase reaction by addition of a chelating agent in a system...
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5258501 |
Stabilization of glycoproteins
A process for the preparation of stabilized glycoproteins without the introduction of foreign molecules. Susceptible monosaccharides covalently attached to the protein part of the molecule undergo...
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5254469 |
Oligonucleotide-enzyme conjugate that can be used as a probe in hybridization assays and polymerase chain reaction procedures
A covalent conjugate of an enzyme, such as peroxidase, glucose oxidase, alkaline phosphatase and beta-galactosidase, and an oligonucleotide is herein disclosed. This conjugate can be used as a...
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5250428 |
L-gulono-gamma-lactone-dehydrogenase for producing vitamin C
The present invention concerns a novel process for the microbiological manufacture of L-ascorbic acid from L-gulono-gamma-lactone, a process for preparing the enzyme being responsible for the...
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5250415 |
Process for the preparation of glucose dehydrogenase from Bacillus megaterium
A process for the preparation of glucose dehydrogenase from Bacillus megaterium. This invention relates to the preparation of a genetically engineered polypeptide with the biological activity of...
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5244796 |
Cloned Leuconostoc mesenteroides glucose-6-phosphate dehydrogenase genes and method of making glucose-6-phospate dehydrogenase
The Glc6PD genome of Leuconostoc mesenteroides is isolated. Oligonucleotides complementary to sequences upstream and downstream to the Glc6PD genome are prepared and used to amplify the genome by...
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5236837 |
Enzymatic reagent for D-malate determination
The invention concerns a new NAD + -dependent D-malate dehydrogenase (decarboxylating, E.C. 1.1.1.83) from E. coli which consists of two subunits which each have a molecular weight of about 42 kD...
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5234827 |
Enzymatic process for manufacturing formaldehyde and hydrogen peroxide
A process is disclosed for enzymatically converting lower alkyl alcohols to corresponding aldehydes and hydrogen peroxide in the presence of oxygen under process conditions which increase the...
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5221289 |
Dyed textile products and a method of producing them
A dyed textile article treated with tannic acid and dyed with an extract of mycelia or basidiocarps of Ganoderma lucidum. This article is produced by a process comprising contacting a substrate...
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5221621 |
Production of glyoxylic acid from glycolic acid
A process or the production of glyoxylic acid involving the enzymatic oxidation of glycolic acid. The process provides a commercially practical method involving the reaction of glycolic acid in an...
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5217880 |
L-fucose dehydrogenase gene, microorganism having said gene and production of L-fucose dehydrogenase by the use of said microorganism
There is provided an isolated gene from Arthrobacter oxidans encoding L-fucose dehydrogenase. Also provided are a process for producing L-fucose dehydrogenase by culturing E. coli containing a...
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