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6153398 |
Method to identify specific inhibitors of IMP dehydrogenase
This invention relates to methods to identify specific inhibitors of the purine nucleotide synthesis enzyme, IMP dehydrogenase (IMPDH). IMPDH is an essential enzyme found in all free-living...
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6146865 |
Nucleic acids encoding polypeptides having pyranose oxidase activity
The present invention relates to isolated nucleic acid sequences encoding polypeptides having pyranose oxidase activity. The invention also relates to nucleic acid constructs, vectors and host...
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6136578 |
Storage-stable liquid formulation comprising a laccase
The present invention relates to a storage-stable liquid formulation comprising a laccase comprising (i) a laccase, (ii) at least one polyalcohol, which formulation has a pH which is more alkaline...
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6127157 |
Coating material
The present invention provides a coating suitable for coating woods, and the coating is obtained by dissolving proanthocyanidine into water or a water-containing alcohol.
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6124124 |
Oxidation in solid state of oxidizable galactose type of alchohol configuration containing polymer
Composition comprising oxidizable galactose type of alcohol configuration containing polymer (such as guar) which is in solid state and galactose oxidase. Application of such oxidized polymers in...
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6114509 |
Polypeptide with reduced allergenicity
The invention relates to modified polypeptides with reduced allergenicity comprising a parent polypeptide with a molecular weight from between 10 kDa and 100 kDa conjugated to a polymer with a...
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6102690 |
Recombinant organisms capable of fermenting cellobiose
This invention relates to a recombinant microorganism which expresses pyruvate decarboxylase, alcohol dehydrogenase, Klebsiella phospho-β-glucosidase and Klebsiella (phosphoenolpyruvate-dependent...
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6103509 |
Modified glucose dehydrogenase
The invention provides a modified pyrrolo-quinoline quinone glucose dehydrogenase (PQQGDH) enzyme protein which is modified to have a substrate specificity to glucose. The modified PQQGDH of the...
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6080915 |
Constructs encoding degradation enzymes for aromatic compounds and transgenic plants containing same
Disclosed is a plant transformation construct comprising DNA having (1) a ring-opening gene that encodes an enzyme that can open an aromatic ring, (2) upstream of the ring-opening gene, a...
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6071704 |
Human aflatoxin B1 aldehyde reductase
The invention provides a human aflatoxin B1 aldehyde reductase (AFB1-hAR) and polynucleotides which identify and encode AFB1-hAR. The invention also provides expression vectors, host cells,...
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6057141 |
DNA encoding the subunit of avian lactate dehydrogenase
The present invention provides a gene encoding the type B subunit protein of avian lactate dehydrogenase. The gene encoding the type B subunit protein of avian lactate dehydrogenase of the present...
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6057140 |
Human scad family molecules
The invention provides human SCAD family molecules (HSFM) and polynucleotides which identify and encode HSFM. The invention also provides expression vectors, host cells, antibodies, agonists, and...
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6046041 |
Method for producing marine-derived compounds and novel compounds produced therefrom
A fortified enzyme preparation of the gorgonian Pseudopterogorgia americana or Pseudopterogorgia elisabethae has been developed which efficiently transforms a variety of precursor molecules, e.g.,...
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6033882 |
Chiral synthesis of 2-hydroxy carboxylic acids with a dehydrogenase
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6033867 |
Fructosyl amino acid oxidase, process for producing the same, and method of assaying amadori compounds using the enzyme
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6022703 |
Expression of DNA sequences derived from nocardioform microorganisms
A cloning vector contains a DNA sequence derived from a Nocardioform microorganism, and is capable of expressing the DNA sequence in a host microorganism. Recombinant cholesterol oxidase is...
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6022717 |
Use of oxidation promoting chemicals in the oxidation oxidizable galactose type of alcohol configuration containing polymer
Process for the oxidation of the oxidizable galactose type of alcohol in oxidizable galactose type of alcohol configuration containing polymer, such as guar, with galactose oxidase in the presence...
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6008006 |
Determination of glycated proteins
The amount of glycated proteins in a sample can be quantified by reacting the sample with first a reagent which is a combination of a protease and a peroxidase and second with a ketoamine oxidase....
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5962253 |
Oxidative decarboxylation of peptides catalyzed by flavoprotein EpiD
A method is described for oxidatively decarboxylating a peptide, comprising combining a peptide with EpiD, wherein the peptide comprises at its carboxy terminus the amino acid sequence X 1 X 2 X...
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5952209 |
Human retinol dehydrogenase type II homolog
The invention provides a human retinol dehydrogenase type II homolog (HRoDH) and polynucleotides which identify and encode HRoDH. The invention also provides expression vectors, host cells,...
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5948121 |
Laccases with improved dyeing properties
The present invention relates to a permanent dyeing composition comprising a) above 0 to 1 mg enzyme protein per ml dyeing composition of microbial laccase, b) one or more dye precursor, and c)...
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5945278 |
Method and system for enhanced production of commercially important exoproteins in gram-positive bacteria
The invention provides a method and expression system for enhancing secretion of hyperproduced homologous and heterologous exoproteins in gram-positive bacteria such as Bacillus sp. The method and...
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5942427 |
N-acetylmannosamine dehydrogenase gene and novel recombinant DNA as well as a method for production of N-acetylmannosamine dehydrogenase
N-acetylmannosamine dehydrogenase gene derived from a microorganism belonging to the genus Flavobacterium, e.g., Flavobacterium sp. No. 141-8 strain and defined by a specific restriction enzyme map...
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5942262 |
Biscuit doughs and biscuits products and methods of producing same
A dough suitable for baking biscuits is presented which comprises a proteinase which is at least partially inactivated by oxidation with an oxidizing agent and a second enzyme which produces the...
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5942424 |
Method for the enzymatic production of hydrogen
The present invention is an enzymatic method for producing hydrogen comprising the steps of: a) forming a reaction mixture within a reaction vessel comprising a substrate capable of undergoing...
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5932463 |
Gluconobacter alcohol/aldehyde dehydrogenase
The present invention relates to a novel alcohol/aldehyde dehydrogenase (hereinafter referred to as AADH), a process for producing the same and a process for producing aldehydes, carboxylic acids...
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5928670 |
Method of inhibiting aldose reductase in vivo with intrinsic inhibitors of aldose reductase
An intrinsic aldose reductase inhibitor is isolated and purified from mammalian cells, such as rat or human kidney cells. The intrinsic aldose reductase inhibitor may be incorporated into...
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5919684 |
Process for the reduction of catalase activity in alcohol oxidase
A process is disclosed for treating compositions of alcohol oxidase to inactivate catalase therein, which comprises aging the composition comprising alcohol oxidase and catalase at a temperature...
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5916759 |
Cholesterol oxidase from Brevibacterium sterolicum
The invention concerns a cholesterol oxidase, a process for the production of a recombinant cholesterol oxidase, a DNA sequence suitable for this process which causes a cytoplasmatic expression of...
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5916786 |
Method for the production of chiral 1,3-aminoalcohols
The disclosure describes a method for the preparation of chiral 1,3-aminoalcohols in high optical purity. The method combines the stereoselective oxidation of a 1,4-diol to a gamma-lactone using an...
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5916785 |
Process of oxidizing alcohols or carboxylic acids with alcohol/aldehyde dehydrogenase from gluconobacter
The present invention relates to a novel alcohol/aldehyde dehydro-genase (hereinafter referred to as AADH), a process for producing the same and a process for producing aldehydes, carboxylic acids...
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5914257 |
Structural gene for membrane-bound aldehyde dehydrogenase
This invention relates to the structural gene for the membrane-bound aldehyde dehydrogenase derived from microorganisms belonging to the genus Acetobacter, said structural gene having a molecular...
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5908924 |
Cloning and expression of the gene encoding thermoanaerobacter ethanolicus 39E secondary-alcohol dehydrogenase and enzyme biochemical characterization
The adhB gene encoding Thermoanaerobacter ethanolicus 39E secondary-alcohol dehydrogenase (2° Adh) was cloned, sequenced, and expressed in Escherichia coli. The 1056 bp gene encodes a...
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5897995 |
Enzymatic production of gluconic acid or its salts
An enzymatic process for the conversion of glucose into gluconic acid, uses concentrated glucose solutions. The process employs a combination of glucose oxidase and catalase enzymes which may be...
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5891669 |
Methods for producing polypeptides in respiratory-deficient cells
The present invention relates to methods of producing a polypeptide, comprising: (a) introducing into a respiratory-defective mutant of a cell (i) one or more first nucleic acid sequences which...
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5888786 |
Method for producing 2-keto-L-gulonic acid
An expression vector containing both a DNA encoding an L-sorbose dehydrogenase and a DNA encoding an L-sorbosone dehydrogenase; a transformant having an ability to produce 2-keto-L-gulonic acid...
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5879921 |
Recombinant expression of a glucose oxidase from a cladosporium strain
The invention is directed to alkaline glucose oxidases comprising novel peptide sequences. Furthermore, the invention relates to methods for producing and using said glucose oxidases.
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5871948 |
Alkaline reagent solution for eliminating ammonia in an assay
The invention relates to an ammonia elimination reagent for use in an enzymatic assay of a biological substance which produces ammonia as the reaction product. The reagent is an alkaline solution...
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5869283 |
Expression cassette operable in a recombinant host
An expression cassette, operable in a recombinant host, comprising a heterologous DNA coding sequence encoding a protein, which is functional, alone or in cooperation with one or more additional...
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5866392 |
Cellobiose oxidase, and enzymatic agent and a process for treating paper pulp
Enzyme exhibiting cellobiose oxidase activity, which enzyme has a relative activity of at least 70% at a pH of about 9 and a temperature of about 50° C. An enzymatic agent comprising cellobiose...
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5866406 |
Oxidase-producing aspergillus niger
To provide increased levels of oxidase, mutants of Aspergillus niger that constitutively synthesize glucose oxidase when grown in a medium having less than 0.1M (molar) glucose are obtained. To...
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5861487 |
Genetic sequences encoding flavonoid pathway enzymes and uses therefor
The present invention relates to a nucleic acid isolate comprising a sequence of nucleotides encoding, or complementary to a sequence encoding, a dihydrokaempferol (DHK) hydroxylating enzyme or...
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5858644 |
Analysis of analytes in biological fluids
A method for detecting an analyte in a sample uses both the specificity of an enzymatic reaction and the separation power of capillary electrophoresis. In general, the method comprises: (1)...
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5858749 |
Bifunctional protein from carrots (Daucus carota) with aspartokinase and homoserine dehydrogenase activities
A bifunctional enzyme has been isolated and purified from carrots which has both aspartokinase and homoserine dehydrogenase activities in the same polypeptide. The enzyme can be used to regulate...
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5855881 |
Mammalian alcohol dehydrogenase and aldehyde dehydrogenase production in plants
Described is a method of producing mammalian ADH in plants and a method of producing mammalian ALDH in plants. The ADH and ALDH is preferably of human origin and preferably human class IV σ ADH...
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5855621 |
Dye transfer inhibition
The transfer of a textile dye from a dyed fabric to another fabric during washing or rinsing is inhibited by adding an enzyme exhibiting peroxidase activity or an enzyme exhibiting a suitable...
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5856156 |
Microbial cholesterol dehydrogenase, process for its production and use
Oxygen-independent cholesterol-converting enzyme obtainable from Actinomycetes or Basidiomycetes which utilizes artificial electron acceptors as well as a method and reagent for the determination...
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5856148 |
Materials and methods for biosynthesis of serine and serine-related products
Engineered DNA encoding 3-phosphoglycerate dehydrogenase (PGD) has reduced sensitivity to inhibition by serine in comparison to wild-type PGD. The DNA encodes PGD which has at least some level of...
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5834280 |
Glucose oxidases obtained from a cladosporium
The invention is directed to alkaline glucose oxidases comprising novel peptide sequences obtained from a strain of Cladosporium, particulary Cladosporium oxysporum, strain CBS 163.94. Furthermore,...
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5834224 |
Electrochemical sensor containing an enzyme linked to binding molecules bound to a noble metal surface
An electrochemical sensor is provided containing a supporting material having a surface of noble metal such as gold or palladium to which is bound an enzyme such as glucose oxidase via binding...
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