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5229284 |
Enzyme immobilzation on a macropermable agglomerate of microporous α- a
Enzymes are immboilized on an inorganic carrier which is a round, macropermeable agglomerate having a mulberry-type structure formed from spherical microporous α-aluminum oxide hydroxide ...
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5223124 |
Method for immobilizing a polypeptide in a polymer and a membrane produced thereby
The present invention relates generally to the immobilization or incorporation of polypeptides, especially enzymes or other bioactive polypeptides into polymeric matrixes, especially polyurethane,...
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5219926 |
Method of covalently bonding biopolymer to a solid hydrophilic organic polymer
A process for covalently bonding biopolymer, such as protein, to an organic polymer surface coated with hydrophilic nonionic polymer having groups reactive with the biopolymer and having a cloud...
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5215905 |
Immobilization of fructosyltransferase on a basic, porous anion-exchange resin
An immobilized fructosyltransferase enzyme is prepared by providing a porous or macroporous anion exchange resin having at least 1.0 m2 /g of internal surface, at least 0.1 ml/g of total porous...
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5208154 |
Reversibly immobilized biological materials in monolayer films on electrodes
Methods and techniques are described for reversibly binding charged biological particles in a fluid medium to an electrode surface. The methods are useful in a variety of applications. The...
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5208153 |
Process for producing streptovaricin C
Disclosed is a method for selecting a mutant strain belonging to the genus Streptomyces which is a hyper-producer of Streptovaricin C superior to those know heretofore. This is accomplished by...
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5206178 |
Membrane affinity concentration immunoassay
Methods for determining the presence and/or concentration of an analyte in a biological fluid sample are disclosed. The methods generally include admixing in solution certain polymer/reactant and...
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5206136 |
Rapid membrane affinity concentration assays
Rapid assays for analytes of interest in a fluid sample utilize a first conjugate of a labelled reactant that specifically binds to the analyte, and a second conjugate that binds to the analyte...
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5206159 |
Polymer particles containing colloidal iron oxide granules for use as a magnetically responsive reagent carrier
Substantially spherical polymer particles containing a colloidally, stably and uniformly dispersed magnetically responsive substance of about 5 nm to about 500 nm in diameter provide a...
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5204451 |
Activating hydroxyl groups of polymeric carriers using 4-fluorobenzenesulfonyl chloride for binding biologically active ligands
The invention relates to a method of binding biologically active organic ligands to hydroxyl groups of polymeric carriers. The method involves bringing 4-fluorobenzenesulfonyl Chloride into...
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5200270 |
Carrier for a biologically active component for immunoassay or enzymatic reaction
A carrier for a biologically active component for immunoassay or enzymatic reaction, which comprises: a) a thermoplastic resin bead having an average diameter of from 0.05 to 20 mm, b) from 1 to...
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5200334 |
Sol-gel encapsulated enzyme
An active biological material encapsulated in a glass is formed using a sol-gel process. A metal alkoxide is mixed with water and exposed to ultrasonic energy at a pH≤2 to form a single phase s...
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5196478 |
Immobilization of ligands by radio-derivatized polymers
This invention relates to radio-derivatized polymers and a method of producing them by contacting non-polymerizable conjugands with radiolysable polymers in the presence of irradiation. The...
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5192663 |
Article having an organic dye and a monolayer of dried mammalian cells and a method for utilizing the article
A method for drying mammalian cells, such as erythrocytes, lymphocytes, leukocytes and platelets onto a solid-phase support for use in solid-phase immunoassays through use of a drying solution and...
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5185255 |
Cell culture method
A cell culture method proliferates microorganisms, particularly fungi such as molds, actinomycetes, animal cells, plant cells and so on to produce useful substances such as antibiotics, enzymes,...
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5183741 |
Integral multilayer element for glucose analysis
An integral multilayer element for chemical analysis utilizing an oxidase enzyme reaction system is prepared having in the following order: (a) a porous spreading layer; (b) an oxygen-permeable,...
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5182201 |
Lipase immobilization without covalent bonding on an amphiphilic support containing lipophilic alkyl chains
A support matrix particularly suitable for the immobilization of biologically active materials acting on lipophilic substrates results from the reaction of a polyamine-impregnated porous particle...
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5182111 |
In vivo delivery of active factors by co-cultured cell implants
Methods and devices are disclosed for the delivery of an active factor from an implanted co-culture of an active factor-secreting cell obtained from a first source and an augmentary...
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5180661 |
Platelet cross matching
A method and kit for typing platelets in whole blood for human leucocyte antigens (HLA). A panel of HLA antigens is made on a microtiter plate. Each well is contacted with serum to be typed and the...
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5177013 |
Preparation of an immobilized lipase having a low water content without drying
Lipase is absorbed on a carrier to provide an immobilized lipase preparation containing 0.5 to 8% water by a process that does not require drying. The process is carried out by providing a solution...
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5177012 |
Biosensor containing immobilized Zymomonas mobilis cells for measuring glucose, fructose and sucrose
A biosensor for the determination of glucose and fructose concentrations is provided. The biosensor is produced by a method which comprises treating Zymomonas mobilis whole cells with an organic...
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5177018 |
Microorganism employed for producing streptovaricin
A method for selecting hyper-producing strains of streptovaricin C by culturing streptomyces spectabilis and separating the asporogenous colonies. The asporogenous colonies are then separately...
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5177005 |
Method for maintaining immobilized glucose isomerase activity during continuous isomerization of glucose to fructose
This invention provides a method for the maintenance of continuous activity of an immobilized enzyme reaction by initially underloading an adsorbent with a desired enzyme, monitoring the activity...
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5173421 |
Cell culture carriers
This invention relates to cell culture carriers consisting of a porous support, at least a part of which surface being coated with a water-insoluble polymer constituted by (meth)acrylic acid ester...
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5173260 |
Beads fused to a test device support
A test device comprising beads with a reagent attached, the beads being physically fused to a support. The support includes a material selected to have a melting temperature significantly lower...
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5171779 |
Use of plasma to immobilize protein on polymeric surfaces
The present invention relates to a method of immobilizing proteins on a polymeric matrix by means of plasma activation and an apparatus and process for the use of such material. The protein mixture...
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5169757 |
Antibodies or antigens bound to a macroporous hydrophobic synthetic polymer cloth for immunological techniques
A device for immunological techniques is prepared containing a macroporous hydrophobic synthetic polymer cloth having antibodies or antigens directly adsorbed therein and directly absorbed and...
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5166064 |
Immobilized of lipase on a cation exchange resin
An immobilized lipase is prepared by adsorbing lipase on a carrier that is capable of retaining 3% or more by weight of water after drying under a reduced pressure of from 0.01 to 1.0 Torr at 70° ...
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5166077 |
Latex for immobilization of physiologically active substances for immuno nephelometry
A latex for immobilization of a physiologically active substance is prepared by copolymerizing a monomer mixture comprising: (a) 100 parts by weight of an aromatic vinyl monome4r; (b) 0.01 to 5...
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5160626 |
Blotting methods using polyaldehyde activated membranes
This invention relates to methods for gel electrophoresis, for sample transfer, for identifying a macromolecule, for blotting including dot blotting, and for colony hybridization, involving...
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5158880 |
Process for preparing solid perfluorocarbon polymer supports having attached perfluorocarbon-substituted ligand or binder
A process for preparing solid perfluorocarbon polymer supports permitting uniform and secure attachment of perfluorocarbon-substituted ligands or binders to carriers is provided utilizing...
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5158868 |
Method of sample analysis
A system for analyzing a biological fluid or the like for a constituent of interest comprises structure defining a sample inlet port, structure defining an analysis region, a measuring system...
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5158881 |
Method and system for encapsulating cells in a tubular extrudate in separate cell compartments
Methods and systems are disclosed for encapsulating viable cells which produce biologically-active factors. The cells are encapsulated within a semipermeable, polymeric membrane by co-extruding an...
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5156963 |
Immobilization of lipase by adsorption on a particulate macroporous resin
Lipase is immobilized by adsorption on a polymethacrylic acid ester resin such as polymethylmethacrylate cross-linked with divinyl benzene. The resin is preferably a particulate, macroporous resin...
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5154808 |
Functional organic thin film and process for producing the same
A functional organic thin film comprising a water-insoluble organic thin film comprising at least one of a nitrene group and a carbene group and a bioactive protein chemically bound to said at...
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5155166 |
Use of 1-(1-pyrrolidinylcarbonyl)pyridinium salts to attach compounds to carboxylated particles and a kit containing same
Useful materials for diagnostic tests, affinity chromatography, enzymatic reactions and immunoassays are prepared by covalently attaching reactive compounds containing reactive amino or sulfhydryl...
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5144008 |
Artificial carrier for immobilization of biological proteins
An artificial carrier for immobilization of biological proteins, which are composed of particles comprising gelatin, an alkali metal metaphosphate and an anionic high-molecular electrolyte, said...
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5141744 |
Insecticide delivery system and attractant
An insecticidal composition in the form of a hydrated macrogel containing at least one species of entomopathogen and a hydrated water retentive compound which acts as a water-reservoir for the...
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5139881 |
Modified polymeric surfaces and process for preparing same
A process for surface modifying a variety of polymeric support surfaces is disclosed in which a predetermined modifying polymer is irreversibly absorbed onto essentially all the surfaces of the...
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5137818 |
Immobilized biocatalysts
Immobilized water-insoluble biocatalysts in particulate form comprise living cells, particularly yeast, dispersed in a cross-linked gelling agent. An enzyme, particularly amyloglucosidase, may be...
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5135720 |
Reaction chamber for amino-terminal sequence analysis of proteins or peptides
A reaction chamber utilizes a sample carrier composed of a magnetic core and a surface coating effective to support sample of protein or peptide. The sample carrier is floated magnetically by means...
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5134072 |
Preparation of a water-soluble modified enzyme by covalently bonding an enzyme to a polyurethane pre-polymer/bisulfite adduct
A water-soluble polyurethane-modified enzyme is prepared by reaction of an enzyme in aqueous solution with an aqueous solution of a water-soluble polyurethane pre-polymer/bisulfite adduct at a pH...
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5132211 |
Gel body and a method for biological sterilization control
The invention relates to a gel body of a delimited physical form preferably containing water and characterized in that it a) contains an encapsulated apathogenic micro-organism having a known and...
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5130237 |
Substrate conversion with an enzyme immobilized on an ultrafiltration membrane
A process is disclosed for chemically converting a substrate into its reaction products and immediately thereafter physically separating the reaction products in a continuous operation. The process...
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5128251 |
Immobilized lipolytic enzyme for esterification and interesterification
An immobilized lipolytic enzyme for esterification and interesterification of fats and oils is prepared by adsorbing a fatty acid or a derivative thereof onto an insoluble carrier and then...
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5122456 |
Amperometric method for the quantitative determination of 1,4-dihydronicotinamide adenine dinucleotide (NADH) in solution
A method is disclosed for the quantitative determination of 1,4-dihydronicotinamide adenisne dinucleotide (NADH) in solution. The method comprises contacting the NADH-containing solution with an...
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5122452 |
Enzyme immunoassay with a macroporous hydrophobic synthetic polymer cloth containing an immobilized antibody or antigen
An immunoassay device containing an immobilized antibody or antigen is provided by directly absorbing and absorbing an unmodified antibody or antigen on and within a woven or non-woven macroporous...
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5120650 |
Method for producing crystalline glucose isomerase
In a process for growing enzyme crystals, small crystals are continuously removed from a crystallizer, dissolved and returned to the crystallizer to maintain a supersaturated state. The method...
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5120420 |
Biosensor and a process for preparation thereof
A biosensor of the invention comprises an insulating base board (1) having formed thereon, in sequence, leads (2, 3, 3'), an electrode system mainly made of carbon (4, 5, 5'), an insulating layer...
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5118609 |
Carrier fleece for use as a reagent carrier from which reagents can be dissolved in immunological analysis
A carrier fleece is prepared for use as reagent carrier from which reagents can be dissolved in as assay such as immunological analysis. The carrier fleece contains from 5 to 60% by weight of...
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