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7824880 |
High sensitivity assay for detection of nucleoside diphosphate production
The present invention provides assays for detecting ADP, GDP and inorganic phosphate. These assays can be used directly to detect the presence of ADP, GDP and inorganic phosphate or can be used as...
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7416856 |
High sensitivity assay for detection of nucleoside diphosphate production
The present invention provides assays for detecting ADP, GDP and inorganic phosphate. These assays can be used directly to detect the presence of ADP, GDP and inorganic phosphate or can be used as...
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7378254 |
Compositions and assays utilizing ADP or phosphate for detecting protein modulators
Described herein are methods which identify candidate agents as binding to a protein or as a modulator of the binding characteristics or biological activity of a protein. Generally, the methods...
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7361286 |
Method of detecting etching end-point
A method of detecting an etching end-point includes the steps of: forming a mask on a pattern area of an etching object; forming an etching indicator on an etching area of the etching object, which...
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7247435 |
Enzymatic fluorometric assay for cAMP and adenylate cyclase
The present invention relates to a method for quickly determining cAMP content or an adenylate cyclase activity in a biological sample containing non-cyclic adenine nucleotides without the use of...
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7238483 |
Method for detecting catalytic activity
This invention has as its object a method for detecting catalytic activity of a sample, characterized in that it comprises: the incubation of a substrate (S) with the sample that may have the...
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7192727 |
Reagent for measuring alanine aminotransferase activity
A reagent for measuring an alanine aminotransferase activity comprising L-alanine, 2-oxoglutaric acid, lactate dehydrogenase, and reduced nicotinamide adenine dinucleotide, characterized by further...
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7083952 |
Nucleotide sequences coding for proteins involved in the biosynthesis of L-serine, an improved method for the microbial production of L-serine and a genetically modified microorganism suitable therefor
The present invention relates to nucleotide sequences of coryneform bacteria, coding for proteins involved in the bio-synthesis of L-serine and to methods for the isolation thereof. The invention...
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7026136 |
Methods of reducing microbial resistance to drugs
The instant methods and compositions represent an advance in controlling drug resistance in microbes. AcrAB-like efflux pumps have been found to control resistance to drugs, even in highly...
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7018807 |
Method and medium for detecting vancomycin-resistant enterococcus
A microbe-specific medium for detection of vancomycin-resistant Enterococci in a test sample within 24 hours and preferably within 18 hours. The testing medium provides a selective growth medium...
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6995004 |
UDP-N-ACETYLGLUCOSAMINE: GALACTOSE-β 1,3-N-ACETYLGALACTOSAMINE-α-R / N-ACETYLGLUCOSAMINE-β 1,3,-N-ACETYLGALACTOSAMINE-α-R (GLCNAC TO GALNAC) β1,6-N-ACETYLGLUCOSAMINYLTRANSFERASE, C2/4GNT
A novel gene defining a novel human UDP-GlcNAc: Gal/Gl cNAcβ 1-3GalNAc αβ1, 6GlcNAc-transferase, termed C2/4GnT, with unique enzymatic properties is disclosed. The enzymatic activity of C2/4GnT is...
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6958148 |
Linkage of agents to body tissue using microparticles and transglutaminase
Methods, products and kits are provided for attaching agents to a body tissue surface via microparticles using endogenous or exogenous transglutaminase. The microparticles have surface available...
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6919076 |
Conjugates of agents and transglutaminase substrate linking molecules
Methods, products, compositions and kits are provided for attaching agents to tissue with a linking molecule in the presence of transglutaminase. The linking molecule and/or agent is a substrate of...
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6790605 |
Methods for obtaining a desired bioactivity or biomolecule using DNA libraries from an environmental source
Disclosed is a process for obtaining an enzyme having a specified enzyme activity derived from a heterogeneous DNA population by screening, for the specified enzyme activity, a library of clones...
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6762026 |
Enzymatic fluorimetric assay of camp and adenylate cyclase
The present invention relates to a method for quickly determining cAMP content or an adenylate cyclase activity in a biological sample containing non-cyclic adenine nucleotides without the use of...
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6756230 |
Quantitative analysis
Amounts of components in a specimen can be analyzed with excellent quantitativity. The analysis includes: measuring an amount of a component to be analyzed in a specimen; measuring an amount of a...
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6750032 |
Method and diagnostic agent for hemostasis diagnosis
The invention relates to a method and to a diagnostic agent for detecting hemostasis disturbances, wherein, as a consequence of blood platelet aggregation, clot formation and/or clot dissolution,...
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6733986 |
Method and agent for determining a deaminase enzymatic activity
A method and compound for detecting and identifying and/or quantifying a deaminase enzymatic activity of a microorganism, according to which an inoculum suspected of containing a microorganism with...
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6673565 |
Enzymatic polymeric sensors
A sensor for detecting an analyte enzyme includes at least one substrate compound and at least one indicator compound selected to produce a measurable change of state as a result of the interaction...
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6645735 |
Reagent for GPT assay
A two reagent-type kit for assaying GPT by acting GPT on L-alanine and α-ketoglutarate in the presence of pyridoxal phosphate, converting the resulting pyruvate into lactate with L-lactate ...
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6632600 |
Altered thermostability of enzymes
Provided is a method of screening gene libraries derived from a mixed population of organisms for a bioactivity or biomolecule of interest. The mixed population of organisms can be a cultured...
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6471959 |
Human transferase
The invention provides three human transferases (HUTRAN) and polynucleotides which identify and encode HUTRAN. The invention also provides expression vectors, host cells, antibodies, agonists, and...
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6465202 |
Method for stabilizing aminotransferase activity in a biological fluid
Methods and compositions for the stabilization of aminotransferase activity of plasma or serum are provided. Such methods will be useful in the accurate determination of tests associated with liver...
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6436658 |
Method for the determination of homocysteine
The invention relates to a method for determining homocysteine concentration in samples in which homocysteine is condensed using an enzyme cystathionine β sythase to form cystathionine. Pyruvate ...
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6426194 |
Homogeneous enzymatic assay for vitamin B6 and improvements in H2S detection
Enzymatic methods to determine the concentration of pyridoxal 5′-phosphate (PLP) in biological fluids are described. The methods of the invention are useful to assess risk for cardiovascular d...
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6365372 |
SNF2 related CBP activator protein (SRCAP)
A protein, SRCAP, is provided. The protein is capable of co-activating CREB binding protein (CBP) mediated transcription, as well as activating transcription without CBP. SRCAP is a Snf2 family...
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6300092 |
Methods of use of a novel lysyl oxidase-related protein
Novel Lor-2 polypeptides, proteins, and nucleic acid molecules are disclosed. In addition to isolated, full-length Lor-2 proteins, the invention further provides isolated Lor-2 fusion proteins,...
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6291200 |
Enzyme-containing polymeric sensors
A sensor for detecting the presence of at least one analyte includes at least one enzyme that is selected to either (i) catalyze a reaction of the analyte to chemically convert the analyte to a...
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6267957 |
Attaching agents to tissue with transglutaminase and a transglutaminase substrate
Methods, products and kits are provided for attaching agents to tissue with a linking molecule in the presence of transglutaminase. The linking molecule and/or agent is a substrate of...
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6203758 |
Micro-circuit system with array of functionalized micro-electrodes
A micro-circuit for performing analyses of multimolecular interactions and for performing molecular syntheses, comprising: (a) a support; (b) at least one micro-electrode attached to the support,...
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5891621 |
Metabolic pathway assay
An in vitro screening assay which identifies enzyme inhibitors and allows for the simultaneous assay of many enzymes. Enzyme, substrate, co-factor, etc. concentrations are optimized so that...
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5885982 |
Use of transglutaminase inhibitor for the treatment of scar tissue
A method of treating or preventing hypertrophic scar tissue in human skin comprising applying topically an effective amount of a non-toxic amine compound that is a transglutaminase inhibitor having...
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5874229 |
Method for avoiding influence of hemoglobin
A method for avoiding influence of hemoglobin, in which the influence is caused by the changes with time in the absorption wavelength of hemoglobin when light absorption of a color reaction of a...
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5858666 |
Apparatus and method of detection employing an AC frequency sensor array
A transducer for detecting biomolecules comprises a bottom and a top unit. The bottom unit has a set of parallel electrically conductive transmission lines thereon and probes attached to selected...
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5843699 |
Rapid microorganism detection method
A rapid screening method for the detection and identification of target microorganisms in a sample which may contain both target microorganisms and competing non-target microorganisms includes the...
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5834226 |
One-step test for aspartate aminotransferase
Novel assay kits and methods are described for the detection and determination of the severity of disease conditions associated with elevated levels of aspartate aminotransferase (AST). The AST...
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5827645 |
Homocysteine assay
The invention relates to a method for assaying homocysteine in a sample such as blood, plasma, or urine, which comprises the steps of contacting the sample with a homocysteine converting enzyme and...
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5804402 |
Reagent
This invention relates to a reagent for enzymatic determination of an analyte concentration in a patient wherein the degree of oxidation of a coenzyme is measured, characterized in that said...
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5783399 |
Chemiluminescent assay methods and devices for detecting target analytes
A method which utilize chemiluminescence for analyte detection and the detection of bacteria on surfaces. The method uses a device comprising a sampling portion made of a first adsorbent material,...
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5770391 |
Reagents and methods for use in bioluminescence
The subject invention pertains to a combination of enzyme activities comprising an ATP-degrading enzyme and one or more enzymes capable of degrading substances, other than ATP, that are substrates...
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5747277 |
Process for detecting microorganisms
Microorganisms in a sample can be detected easily and rapidly with high precision by a process which comprises filtering the sample through a filter, washing the filter, reacting the residue on the...
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5741659 |
Rapid microbial protease assay
An assay for detecting microbial protease activity in clinical and laboray samples is described which comprises gathering a sample suspected of containing certain microorganisms having the desired...
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5686260 |
Method and composition for the enzymic determination of aspartame
The invention relates to a method and a composition for the enzymic determination of aspartame in aqueous solutions. The method is carried out such that the sample solution is incubated essentially...
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5667984 |
Sequencing of oligosaccharides
A process suitable for use in the sequencing of an oligosaccharide compound includes a first analysis of a primary oligosaccharide compound's monosaccharide composition, selection of sequencing...
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5654152 |
Method of measuring enzyme activity by using a column having an immobilized substrate
Enzyme activity is measured promptly with a high accuracy by introducing an enzyme, the activity of which is to be measured, into a column comprising a hollow tube packed with a filler comprising a...
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5633143 |
Method for the quantitative determination of D-3-hydroxybutyric acid and acetoacetic acid, and analytical reagent therefor
Disclosed is a method for the quantitative determination of D-3-hydroxybutyric acid and acetoacetic acid, which comprises reacting a biological sample containing D-3-hydroxybutyric acid and...
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5618684 |
Method of determination of calcium
Calcium in a sample is brought into contact with a transglutaminase capable of being activated with calcium as an activating factor and the transglutaminase activity, which varies depending upon...
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5605795 |
Assays using chemiluminescent, enzymatically cleavable substituted 1,2-dioxetanes and kits therefor
Dioxetane compounds reactable with an enzyme to release optically detectable eneregy are disclosed. These compounds have the formula: ##STR1## wherein T is a cycloalkyl group or a fused...
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5585248 |
Method for assaying activity of prophenoloxidase activating enzyme and application thereof
A method for assaying an activity of a prophenoloxidase activating enzyme (PPAE), comprising assaying at least X-Arg or Y produced upon contact of the PPAE with a peptide chain of the formula...
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5580747 |
Non-radioactive enzyme assay
The present invention is directed to the assay and purification of proteins, and particularly to the non-radioactive assay and purification of protein kinases, phosphatases and protease by...
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