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7135315 |
Aldehyde dehydrogenase gene
The present invention relates to a novel DNA which encodes aldehyde dehydrogenase (SNDH), an expression vector containing the DNA and recombinant organisms containing said DNA. Furthermore, the...
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7109004 |
Process for reducing an alpha-keto ester
A process for preparing (R)-hydroxy ester (II)
from alpha-keto ester I
comprising adding the alpha-keto ester I to a mixture comprising the ketoreductase enzyme and non-ketoreductase enzyme...
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7091013 |
Process for the manufacture of 2-keto-L-gulonic acid
The present invention discloses a process for producing sodium 2-keto-L-gulonate from sorbitol and recovering 2-keto-L-gulonic acid in high recovery yields, by controlled cation exchange treatment...
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7033804 |
Methods for the production of products in host cells
The invention provides methods and host cells for the production of ascorbic acid intermediates. The invention also provides host cells having a modification in a polynucleotide that uncouples the...
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6824996 |
Production of ascorbic acid
The present invention provides for the production of ASA from yeast capable of producing ASA from KLG. The present invention provides methods for the production of ASA as well as recombinant yeast...
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6730503 |
Alcohol/aldehyde dehydrogenase
The present invention is directed to a recombinant enzymes having alcohol and aldehyde dehydrogenase activity which comprises one or mom recombinant polypeptides selected from the group consisting...
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6670505 |
Process for the recovery of organic acids from aqueous solutions
A process for recovering a desired organic acid from a solution includes the steps of: providing an aqueous solution including at least one desired organic acid or its acid anion; adjusting the...
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6399340 |
Vector derivatives of gluconobacter plasmid pF4
A DNA derived from plasmid pF4, which contains a region involved in the control of autonomous replication in a bacterial cell belonging to the genus Gluconobactor, with or without a part of the...
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6271006 |
Enzymatic process for the manufacture of ascorbic acid, 2-keto-L-gulonic acid and esters of 2-keto-L-gulonic acid
The present invention is directed toward efficient, high-yield processes for making ascorbic acid, 2-keto-L-gulonic acid, and esters of 2-keto-L-gulonic acid. The processes comprise reacting the...
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6238897 |
Continuous process for producing 2-keto-L-gulonic acid
A process for the continuous production of 2-keto-L-gulonic acid or a salt thereof from D-sorbitol by fermentation with microorganisms in a nutrient medium containing D-sorbitol that is incubated...
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6187570 |
Electrodialysis methods for purification and recovery of gluconic acid derivatives
A process for purifying and concentrating a gluconic acid derivative, such as 2-keto-L-gulonic acid, comprising introducing a non-viable and/or acidified fermentation medium or an in-vitro reactor...
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5998180 |
Nitrilase from Rhodoccus rhodochrous for converting acrylonitrile directly to acrylic acid
Nitrilase enzymes are provided which have Km at pH 7.0 for acrylonitrile of 500 μM or below. The enzymes also have Ki at pH 7.0 for ammonium acrylate of at least 100 mM. In particular, the...
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5989891 |
Bacterial stains and use thereof in fermentation processes for 2-keto-L-gulonic acid production
The present invention relates to a process for the production of 2-keto-L-gulonic acid by fermentative conversion of L-sorbose and/or D-sorbitol. The present invention further relates to novel...
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5932463 |
Gluconobacter alcohol/aldehyde dehydrogenase
The present invention relates to a novel alcohol/aldehyde dehydrogenase (hereinafter referred to as AADH), a process for producing the same and a process for producing aldehydes, carboxylic acids...
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5916785 |
Process of oxidizing alcohols or carboxylic acids with alcohol/aldehyde dehydrogenase from gluconobacter
The present invention relates to a novel alcohol/aldehyde dehydro-genase (hereinafter referred to as AADH), a process for producing the same and a process for producing aldehydes, carboxylic acids...
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5900370 |
Process for the production of ascorbic acid with prototheca
The present invention is directed to methods for the production of ascorbic acid by culturing organisms of the genus Prototheca and recovering ascorbic acid from the fermentation medium.
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5888786 |
Method for producing 2-keto-L-gulonic acid
An expression vector containing both a DNA encoding an L-sorbose dehydrogenase and a DNA encoding an L-sorbosone dehydrogenase; a transformant having an ability to produce 2-keto-L-gulonic acid...
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5834231 |
Bacterial strains and use thereof in fermentation process for 2-keto-L-gulonic acid production
The present invention relates to a process for the production of 2-keto-L-gulonic acid by fermentative conversion of L-sorbose and/or D-sorbitol. The present invention further relates to novel...
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5804423 |
Microbiological method of making 5-ketogluconate
A method is disclosed for preparing 5-ketogluconate, which comprises the steps of: (a) genetically modifying a microorganism capable of microbiologically producing 5-ketogluconate to increase...
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5795761 |
Mutants of 2,5-diketo-D-gluconic acid (2,5-DKG) reductase A
Mutants of 2,5-diketo-D-gluconic acid reductase A an enzyme used to produce 2-keto-L-gluconic acid, a precursor of ascorbic acid (vitamin C), are prepared by site-directed mutagenesis. These...
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5792631 |
Microbial process for the production of ascorbic acid using Chlorella protothecoides
The present invention is directed to methods for the production of ascorbic acid by culturing organisms of the species C. protothecoides and recovering ascorbic acid from the fermentation medium.
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5776742 |
Aldehyde dehydrogenase enzyme
An aldehyde dehydrogenase having the physico-chemical properties: molecular weight: 91,000±5,000; substrate specificity:active on aldehyde compounds; inhibition: by Cu 2 +, Zn 2 +, Ni 2 + and...
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5759826 |
Process of preparing an organic acid
A process is disclosed for preparing an organic acid and/or its salts from a solution obtained through fermentation. In this process, a pure carbohydrate-containing raw material is used. The...
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5747306 |
Process for producing 2-keto-L-gulonic acid using electrodialysis
In a microbial process for producing 2-keto-L-gulonic acid (hereinafter sometimes referred to as 2KGA) by fermentation or microbial cell reaction with at least one microorganism capable of...
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5705373 |
Production of 2-keto-L-gulonic acid using pseudogluconobacter saccharoketogenes with recycling
A method for producing 2-keto-L-gulonic acid (hereinafter referred to as 2KGA) which comprises (1) culturing a microorganism capable of producing 2KGA from L-sorbose, in a liquid culture medium...
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5635611 |
Production of saccharide carboxylic acids
A process for producing a saccharide carboxylic acid or a salt thereof characterized in that a microorganism belonging to the genus Pseudogluconobacter and capable of oxidizing a hydroxymethyl...
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5541108 |
Gluconobacter oxydans strains
A process for producing 2-keto-L-gulonic acid which comprises converting L-sorbose and/or D-sorbitol into 2-keto-L-gulonic acid with the aid of a microorganism or its cell free extract, said...
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5521090 |
L-ascorbic acid containing biomass of chlorella pyrenoidosa
Microalgal biomass that comprises cells of Chlorella pyrenoidosa which contain greater than 2.0% by dry weight of L-ascorbic acid (Vitamin C), microorganisms and processes that form the biomass,...
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5474924 |
Method for producing 2-keto-L-gulonic acid
2-keto-L-gulonic acid is in high yield produced by contacting a microorganism of the genus Pseudogluconobacter, either as it is or after processing, with L-sorbose.
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RE34851 |
Biosynthesis of 2 keto-L-gulonic acid
A process of producing 2-keto-L-gulonic acid from sorbose via a recombinant bacteria including expression vectors and probes for producing said recombinant bacteria.
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5352599 |
Co-enzyme-independent L-sorbosone dehydrogenase of gluconobacter oxydans: isolation, characterization, and cloning and autologus expression of the gene
A novel coenzyme independent L-sorbosone dehydrogenase originating from a microorganism belonging to the genus Gluconobacter oxydans which acts on L-sorbosone to produce 2-keto-L-gulonic acid. The...
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5312741 |
Process for producing 2-keto-L-gulonic acid
The present invention relates to a process for producing 2-keto-L-gulonic acid by fermentative conversion of D-sorbitol in high yield. 2-Keto-L-gulonic acid is an important intermediate for the...
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5234819 |
Method for preparing 2,5-diketo-D-gluconic acid
2,5-Diketo-D-gluconic acid is prepared in high yield and in high broth concentration by cultivating newly isolated microorganisms of genus Erwinia in an aqueous nutrient medium in the presence of...
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5134077 |
Microorganisms of the genus Erwinia useful for preparing 2,5-diketo-D-gluconic acid
2,5-Diketo-D-gluconic acid is prepared in high yield and in high broth concentration by cultivating newly isolated microorganisms of genus Erwinia in an aqueous nutrient medium in the presence of...
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5085993 |
Coenzyme-independent L-sorbosone dehydrogenase from Pseudomonas putida
A coenzyme-independent L-sorbosone dehydrogenase has been prepared from a microorganism belong to the genus Pseudomonas, which acts on L-sorbosone to produce 2-keto-L-gulonic acid. The enzyme is...
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5077206 |
Process for preparing L-rhamnose
The invention provides a process for preparing L-rhamnose by hydrolyzing a rhamnosidic bond of a glycoside having rhamnose in a terminal position, by enzymatically hydrolyzing the glucoside with an...
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5032514 |
Metabolic pathway engineering to increase production of ascorbic acid intermediates
In recombinant microorganisms which were rendered capable of converting 2,5-diketo-D-gluconic acid (2,5-DKG) to 2-keto-L-gulonic acid (2-KLG) by transfer of genetic material, the secondary...
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5008193 |
Ascorbic acid intermediates and process enzymes
Methods for producing ascorbic acid using recombinant means comprising the transfer of genetic material by conjugation, a host cell lacking, entirely or to such an extent as not to be commercially...
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5004690 |
Ascorbic acid intermediates and process enzymes
A process for the preparation of 2-keto-L-gulonic acid (2-KLG) from 2,5-diketo-D-gluconic acid (2,5-DKG) using a DNA sequence encoding 2,5-DKG reductase. The 2-KLG is a precursor for the synthesis...
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5001059 |
L-ascorbic acid production in microorganisms
Improved production of ascorbic acid is obtained empolying Chlorella as a microorganism source and growing the culture under a controlled pattern of carbon source supply. Greatly improved ratios of...
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4990441 |
Process for separating 2-keto-L-gulonic acid from a fermented medium
2-Keto-L-gulonic acid is separated from a fermented medium containing the calcium salt of 2-keto-L-gulonic acid, by carrying out the following operations: separation of insolubles; removal of...
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4960695 |
Fermentation process to produce 2-keto-L-gluonic acid
A process of producing 2-keto-L-gulonic acid by fermentative conversion of L-sorbose utilizing a fermentation system composed of component produced from a microorganism having the identifying...
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4945052 |
Production of a Vitamin C precursor using genetically modified organisms
An enzyme for conversion of 2,5-diketo-D-gluconate (2,5-DKG) to 2-keto-L-gulonic acid (2-KLG) and a genetically modified organism that expresses all the fermentation enzymes needed to convert...
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4935359 |
Fermentation process
Disclosed is a fermentation process for the conversion of L-sorbose to 2-keto-L-gulonic acid, which is characterized in that a mixed culture of microorganisms is used, which comprises Gluconobacter...
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4933289 |
Biologically pure cultures of Pseudomonas sorbosoxidans useful for producing 2-keto-L-gulonic acid
2-Keto-L-gulonic acid is produced in a high yield by contacting L-sorbose with a microorganism of Pseudomonas sorbosoxidans, or a processed material thereof.
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4916068 |
Bioconversion production of ascorbic acid with L-galactono-1,4-oxidase
Processes for the bioconversion production of L-ascorbic acid (Vitamin C), and to microorganisms (e.g., Candida Norvegensis MF-56, ATCC 20686 and Candida Norvegensis MF-78, ATCC 20732) and...
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4892823 |
Method for producing 2-keto-L-gulonic acid
2-Keto-L-gulonic acid is produced in a high yield by contacting L-sorbose with a microorganism of Pseudomonas sorbosoxidans, or a processed material thereof.
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4877735 |
Process for producing 2-keto-L-gulonic acid
An improved process for producing 2-keto-L-gulonic acid which comprises culturing a microorganism belonging to the genus Pseudogluconobacter which has an ability to oxidize L-sorbose to...
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4876195 |
Process for producing 2-keto-D-glucaric acid
2-Keto-D-glucaric acid is produced in high yield by contacting raw material saccharides (e.g. D-glucose) with a bacterium belonging to the genus Pseudogluconobacter or processed matters thereof.
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4857469 |
Process for preparing optically active mercapto compound
A process for preparing an optically active mercapto compound having asymmetrical centers having the formula: ##STR1## in which R 1 represents an alkyl group, R represents a hydrogen atom or an...
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