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7354702 |
Processing tissue to produce a biopolymer scaffold for tissue engineering
A method of forming and preserving a bioremodelable, biopolymer scaffold material by subjecting animal tissue, particularly fetal or neo-natal tissue, to chemical and mechanical processing. The...
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7318998 |
Tissue decellularization
The present invention relates, in general, to tissue decellularization and, in particular to a method of treating tissues, for example, heart valves, tendons and ligaments, so as to render them...
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7316896 |
Egg freezing and storing tool and method
An egg freezing and storing instrument has an egg freezing and storing tube made of a liquid nitrogen-resistant material; and a metal cylindrical protection member for protecting the tube. The tube...
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7314755 |
Preservation of eukaryotic cells using reversible pore formation
A method is disclosed for reliably stabilizing eukaryotic cells that express the P2X 7 receptor channel, particularly mammalian and other vertebrate cells, including human cells, for example...
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7270946 |
Method for treatment of cellular materials with sugars prior to preservation
Living cellular material may be preserved by incubating the cellular material in a culture medium containing at least one sugar, particularly for at least three hours, and then subjecting the...
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7256040 |
Method and apparatus for preparing monolayers of cells
The invention concerns an apparatus for preparing monolayers of cells. The apparatus comprises a container ( 6 ) for a cryosubstitution system and an insert ( 1 ) for the container, the insert ( 1...
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7229820 |
Apparatus and method for culturing and preserving tissue constructs
A disclosure is made of various apparatus and methods for culturing and preserving cells and tissue in ways that minimize contamination potential, direct cells to reside in desired areas, allow...
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7176022 |
Directly injectable formulations which provide enhanced cryoprotection of cell products
This invention provides compositions and methods for cryoprotection of recombinant live cancer cells. Specifically, an improved cryoprotective medium is provided which includes a hydroxyethyl...
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7157222 |
Method of cryopreservation of tissues by vitrification
A method for vitrification of a tissue or organ includes immersing the tissue or organ in increasing concentrations of cryoprotectant solution at a temperature greater than −15° C. to a...
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7153472 |
Preservation and formulation of bioactive materials for storage and delivery in hydrophobic carriers
This invention relates to formulations comprising biological samples preserved as dry glassy powders and hydrophobic carriers, the formulations being adapted for the long-term storage and delivery...
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7138227 |
Frozen spermatozoa compositions and uses thereof
The present invention provides a composition for freezing or freeze-drying spermatozoa or sperm heads thereof, wherein the composition enables the spermatozoa or sperm heads thereof to maintain...
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7129035 |
Method of preserving tissue
This invention relates to the sterilization and preservation of tissue for storage. More specifically, the invention relates to a method of using a solution to form a preserved tissue. The solution...
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7094601 |
Microinjection of cryoprotectants for preservation of cells
A preservation method for biological material having cell membranes includes microinjecting the cells with sugar; preparing the cells for storage; storing the biological material; and recovering...
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7090974 |
Ultrasound-mediated high-speed biological reaction and tissue processing
Methods of fixing and processing tissue and samples on a membrane by using ultrasound radiation as a part of the method are presented. Ultrasound of a frequency in the range of 0.1–50 MHz is used...
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7087370 |
Kit for vitrifying specimens
The present invention relates to systems for the vitrification of a biological specimen. The systems employ a transfer instrument such as a loop, containing a base medium that includes a...
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7029839 |
Methods and solutions for storing donor organs
A method of preserving, storing and transplanting mammalian donor organs. The method includes the cooling of refrigeration preservation, loading pre-freezer preservation, cryopreservation and...
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7011937 |
Method and solutions for cryopreserving oocytes, especially fresh human oocytes
In a method for cryopreserving fresh human oocytes, freezing and thawing solutions are used, which solutions include 1,2 propanediol (PROH) and sucrose at a concentration of at least 0.3M. The...
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6991787 |
Methods of preparing bone marrow stromal cells for use in gene therapy
This invention relates to sequential methods of cryopreserving bone marrow stromal cells that are transfected and used for gene therapy by transplantation. These methods include the following steps...
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6982172 |
Oocyte vitrification technique
A new method of cryopreservation based on the very fast cooling rates achieved by the direct contact of small droplets of vitrification solution containing biological sample with a very cold solid...
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6962775 |
Wound repair dressings and methods for their preservation
Methods and products relating the preservation of cultured mammalian epithelial or mesenchymal cells are provided. The methods involve the pre-treatment of epithelial cells with a solution...
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6962774 |
Method for dry-preserving multicellular organism tissue at ordinary temperatures
A tissue of a multicellular organism is gradually dried during cultivation. After the tissue has been completely dehydrated, water is added to the tissue for its recovery. The tissue of the...
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6953655 |
Compositions, methods and devices for maintaining an organ
Compositions, methods, systems/devices and media are provided for maintaining a harvested organ in a functioning and viable state prior to implantation. The organ perfusion apparatus includes a...
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6951712 |
Cryoprotective system comprising polymeric nano- or micro-particles
The present invention is directed to a cryoprotective system that comprises an aqueous solution containing polymeric nano- and micro-particles that exhibit a reversible temperature-dependent volume...
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6949335 |
Polyglycerol and lactose compositions for the protection of living systems from states of reduced metabolism
Polyglycerol, lactose, and a combination of polyglycerol and lactose are effective at preserving cells, tissues, and organs from damage due to hypothermic, ischemic, or other metabolic impairment,...
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6946241 |
Physiological medium for perfusing, preserving and storing isolated cell, tissue and organ samples
A physiological liquid medium is provided having the basic, synergistic components to allow its universal application in preserving cellular and functional integrity in vitro of different cell,...
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6942961 |
Method for dehydrating biological tissue for producing preserved transplants
The invention relates to a two-step method for dehydrating biological tissues for producing preserved transplants. In a first step, the tissue is partially dehydrated with an organic,...
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6933103 |
Biocompatible tissue for therapeutic use and method of making same
A biocompatible graft material and a method for making the same are disclosed. The method of making the graft material involves freezing and subsequently thawing a donated tissue sample in a bleach...
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6921633 |
Methods and compositions for the preservation of cells, tissues or organs in the vitreous state
The invention provides methods for the hypothermic preservation of cells, tissues and organs. In particular, the invention provides methods for the hypothermic preservation or storage of cells,...
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6916602 |
Methods of preserving functionality of an ovary, preserving fertility of a patient undergoing a treatment expected to cause sterility and assuring a supply of viable gametes for future use
A method of preserving functionality of an organ. The method includes removing a whole organ and associated vasculature, cryo-preserving the whole organ, allowing a period of time to elapse,...
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6902931 |
Controlled reversible poration for preservation of biological materials
A preservation method for biological material having cell membranes includes reversibly porating the cell membranes; loading a bio-protective agent having bio-preservation properties to a...
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6900009 |
Method for creating a frozen tissue array
The present invention provides a method for creating a frozen tissue array. An oil in a liquid form is added into a recipient block containing frozen tissue cores where the oil has a freezing point...
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6872357 |
Formulation of preservation mixtures containing sensitive biologicals to be stabilized for ambient temperature storage by drying
This invention relates to formulations and methods for preserving sensitive biologicals, viruses, bacteria and eukaryotic cells by drying. More particularly, the invention relates to preservation...
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6869758 |
Method and device for removal of cryoprotectant from cryopreserved biological cells and tissues
Novel methods and devices for removing cryoprotectant from cryoprotectant-containing liquids, and from cells residing therein, are disclosed. In one aspect, the method comprises passing the...
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6869757 |
Advantageous carrier solution for vitrifiable concentrations of cryoprotectants, and compatible cryoprotectant mixtures
Disclosed herein is a carrier solution for cryoprotectants that is useful for use with cells, tissues, and whole organs and for a variety of cryoprotectant solutions and that permits antinucleators...
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6858424 |
Cryopreservation vial apparatus and methods
Disclosed is a biopharmaceutical product cryopreservation system, for cryopreserving a biopharmaceutical product that includes a cryopreservation compartment; a cryopreservation fluid located...
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6821779 |
Methods for storing neural cells
The instant methods pertain to improved methods for storing neural cells, preferably dissociated neural cells, prior to their use in transplantation and to the cells obtained using such methods....
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6794124 |
Preservation solution
An improved preservation solution is described, which is intended for the preservation of organs and tissues, or parts thereof, from humans and animals. The improved preservation solution contains...
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6790603 |
Compositions for the storage of platelets
Blood platelets stored in an isotonic, balanced salt solution under standard storage conditions retain function after ten days of storage when micormolar or nanomolar amounts of pentose are added...
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6773877 |
Methods of using ice-controlling molecules
A dispersal pattern of hydrogen bonding sites on an ice surface is used as a template in a process for the design, selection and manufacture of synthetic ice interface dopants. Ice interface...
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6758362 |
Specimen holders for hydrous specimens and method of using them
A specimen holder for a hydrous specimen comprising: (a) an inner hollow cylinder of a heat conductive material, (b) an inner hollow cylinder of a material which can be cut, (c) a cylindrical...
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6743575 |
Compositions and methods for the preservation of living tissues
The present invention provides solutions and methods for preserving living biological materials that enable organs, tissues and cells to be stored for extended periods of time with minimal loss of...
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6713245 |
Methods for storing neural cells such that they are suitable for transplantation
The instant methods pertain to an improved methods for storing neural cells, preferably dissociated neural cells, prior to their use in transplantation and to the cells obtained using such methods....
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6696238 |
Transplant media
The present invention relates to media containing purified antimicrobial polypeptides, such as defensins, and/or cell surface receptor binding proteins. The media may also contain buffers,...
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6682931 |
Recovering cryopreserved conifer embryogenic cultures
This invention relates to a method for improving the growth and regeneration potential of embryogenic cell and tissue cultures of coniferous plants retrieved from cryopreservation. In particular,...
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6673607 |
Microinjection of cryoprotectants for preservation of cells
A preservation method for biological material having cell membranes includes microinjecting the cells with sugar; preparing the cells for storage; storing the biological material; and recovering...
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6646238 |
Method and device for a accomodating samples on cryosubstrates
For sample picking on a cryosubstrate, on which multiple cryopreserved samples are each positioned at preselected sample positions, individual samples are selectively separated mechanically or...
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6641992 |
Aqueous solution for preserving tissues and organs
An aqueous solution for preserving tissues and organs and methods of use for the same are disclosed.
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6638709 |
Processes for making cryopreserved composite living constructs and products resulting therefrom
Processes are described for making a cryopreserved Composite Living Construct (CCLC) as well as a corresponding thawed and rinsed CCLC, comprised of separated layers of cultured fibroblasts and...
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6635414 |
Cryopreservation system with controlled dendritic freezing front velocity
A biopharmaceutical material cryopreservation system is provided that includes a flexible sterile container comprising a biocompatible polymeric material. The flexible sterile container contains...
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6616858 |
Prevention of ice nucleation by polyglycerol
Linear polymers of glycerol can prevent or delay ice nucleation in a variety of contexts. Polyglycerol can also be employed in combination with other ice control agents, such as polyvinyl...
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