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7569130 |
Fluorescent detection of proteins in polyacrylamide gels
The mechanism of the UV light-induced reaction between the indole moiety of tryptophan and chloroform, and the structure of the modified tryptophan and polypeptides including such modified...
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7531073 |
Sparsely cross-linked nanogels: a novel polymer structure for microchannel DNA sequencing
The present invention is generally directed to novel polymeric materials for use in the electrophoretic separation of nucleic acids. In particular, the novel polymer materials are sparsely...
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7473341 |
Denaturant-free electrophoresis of biological molecules under high temperature conditions
The present invention relates to a method of separating a sample comprising biological compounds, such as nucleic acids. The nucleic acids are subjected to electrophoresis using a matrix that is...
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7326324 |
Purification and use of gellan in electrophoresis gels
Gellan can be purified from nucleic acid contamination by combining the contaminated gellan with DNase under conditions that allow the DNase to degrade the nucleic acid contaminant. The purified...
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7320747 |
Gel for electrophoresis
The present invention is directed to a solidified hybrid gel for use in an electrophoresis process, having a solidified first gel portion juxtaposed with a solidified second gel portion. The first...
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7255781 |
Production of aligned microfibers and nanofibers and derived functional monoliths
The present invention comprises a method for producing microfibers and nanofibers and further fabricating derived solid monolithic materials having aligned uniform micro- or nanofibrils. A method...
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7217348 |
Methods of identifying kinetically stable proteins
The present invention provides a fast and efficient means for identifying kinetically stable proteins. As used herein the term “kinetically stable protein” means a protein that is trapped in a...
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7147762 |
Method of electrophoresis
The present invention relates a method of electrophoretic separation of protein and/or peptide components of a sample in a convection stabilized medium. More specifically, the method comprises the...
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7118900 |
Directed assembly of functional heterostructures
The present invention relates to a systematic process for the creation of functionally organized, spatially patterned assemblies of polymer-microparticle composites including the AC electric...
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7083711 |
Capillary electrophoresis gel especially for separation made for single stranded nucleic acid separations
A separation matrix for capillary electrophoresis of single-stranded nucleic acids is disclosed. The matrix comprises an aqueous buffer containing a denaturant and (hydroxypropyl)methyl cellulose.
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7056426 |
Pre-cast electrophoresis slab gels with extended storage life
In pre-cast slab gel cassettes, the formation of pathways in which proteins can migrate between the gel and the walls of the cassette to form shadow bands is avoided by including a nonionic...
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7052590 |
Method and kit for the manufacture of separation gels
A method for the manufacture of a separation gel, which comprises the steps: (i) providing a polymerization mixture containing a first monomer (I) having a polymerizable unsaturated structure and a...
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6942775 |
Vertical electrophoresis system
A plugging medium is used to block the bottom opening of cassettes for vertical electrophoresis in order to facilitate filling the cassettes with separation media. Cassettes can be filled within a...
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6939453 |
Electrophoresis process using ionic liquids
Highly hydrophobic compounds and hydrophobic proteins are solubilized in a non-aqueous solvent containing an electrolyte for electrophoretic separation. The non-aqueous solvent is an ionic liquid...
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6878254 |
Size separation of analytes using monomeric surfactants
A sieving medium for use in the separation of analytes in a sample containing at least one such analyte comprises a monomeric non-ionic surfactant of the of the general formula, B-A, wherein A is a...
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6830670 |
Heat-sensitive medium for separating species in a separating channel and use thereof
A medium comprises an electrolyte wherein is dissolved at least an assembly of block copolymers characterized in that the block copolymers: are present in the electrolyte at a concentration level...
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6818718 |
Electrophoresis gels and cross-linking agents for their preparation
Method of separating molecules by providing a cross-linked polymer gel having a cross-linking moiety of the formula wherein X, X′, Y, Z and R 2 are as defined in the specification. A sample...
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6787016 |
Dynamic coating with linear polymer mixture for electrophoresis
Compositions and methods are provided for performing capillary electrophoresis using a composition comprising in combination in an aqueous buffered medium a coating polymer and a sieving polymer,...
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6726821 |
Polyacrylamide precast gels for electrophoresis, process for producing the same and electroporesis method by using the gels
A precast acrylamide electrophoresis gel is disclosed, which includes a gel buffer solution containing either Tris and glycine or Tris, glycine and any one or more than two of ampholytes, and a...
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6616825 |
Electrochromatographic device for use in enantioselective separation, and enantioselective separation medium for use therein
An electrochromatographic device is provided for conducting enantioselective separation of enantiomers. The device is comprised of a conduit containing a monolithic enantioselective separation...
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6590096 |
Process for the production of polysaccharide beads
The present invention relates to a method for producing porous polysaccharide beads, comprising: (a) mixing a water-based solution of a polysaccharide under stirring with an essentially...
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6585873 |
Polymer gels and methods for their preparation
A crosslinked hydrophilic gel in which the crosslinker used in a compound of Formula I or Formula II or combination thereof. These crosslinked hydrophilic gels provide enhanced separation in...
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6464850 |
Method for producing hydrophilic monomers and uses thereof
The invention relates to a method for producing hydrophilic monomers which are particularly useful for electrophoresis and to electrophoresis compositions and coating compositions. The...
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6391176 |
Detection of alcaline isophosphatases by electrophoresis
The invention concerns a process for separating alkaline phosphatase (ALP) isoenzymes from a biological sample by electrophoresis, characterized in that the electrophoresis is carried out on an...
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6365415 |
Method for characterization and quality control of porous media
The present invention provides a method to characterize, optimize, and control the quality and production of hydrogel media. Additionally, the present invention provides a method for determining...
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6319380 |
Use of an earlier known separation material and novel forms of the material
The use of a gel produced by polymerization of a dextran derivative which exhibits groups that contain an alkene structure, for separating nucleic acid by electrophoresis.
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6261430 |
Micro-electrophoresis chip for moving and separating nucleic acids and other charged molecules
A microelectrophoresis chip comprises a substrate in which there are formed one or more channels, one channel for each sample to be evaluated. The channels extend for the length of the chip, a...
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6203680 |
Electrophoresis gels
The present invention provides electrophoresis apparatus and electrophoresis methods employing gellan gum based gels employing divalent metal cation and diamine cross-linking agents. The gels are...
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6197173 |
Methods and reagents for gel electrophoresis
Polymerization of gels for electrophoresis with improved photoinitiators results in gels which are suitable for electrophoresis. The new initiator systems are much faster than current systems for...
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6013166 |
Method for reducing the linear dimension necessary for high resolution electrophoretic separation
The present invention relates to an electrophoretic method to effect differential net migration, the extent of said migration being dependent on molecular size, of electrically charged...
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5968332 |
Discontinuous and nonsequential polymeric gel systems for separation of macromolecules
This invention relates to the formation and use of a variable, multilayer system of discrete, discontinuous and nonsequential molecular sieves, comprising of agarose-acrylic gels of distinct...
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5964999 |
Methods for profiling oligosaccharides released from glycoproteins
Methods for profiling oligosaccharides released from glycoproteins and methods for the electrophoretic separation of high mannose, complex and hybrid oligosaccharides are disclosed. The methods...
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5961801 |
DNA separation electrophoresis gels and methods for their use
A novel electrophoresis separation medium comprises polyacrylamide and a dioxane. An electrophoresis separation system comprises a separation channel, the separation channel including the novel...
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5928484 |
Separation procedure for Lp(a) by means of electrophoresis, gels for the implementation of this procedure, and application to the in vitro determination of the atherogenic risk associated with the presence of Lp(a)
The invention relates to a separation procedure for Lp(a) and the various lipoproteins contained in a biological sample by electrophoresis. The present invention is characterized in that the...
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5925229 |
Low density lipoprotein fraction assay for cardiac disease risk
A variable rate density gradient electrophoric gel is described which separate LDL subfractions with the precision of ultracentrifugation techniques. Also, an innovative bottom inlet mixing chamber...
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5888365 |
Methods for the separation of biological materials
In accordance with the present invention, there are provided methods for the separation of biological materials employing a variety of polymeric materials with defined chemical, physical and...
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5885432 |
Un-crosslinked polymeric media for electrophoresis
Electrophoretic media are provided comprising at least un-crosslinked polymers which have a temperature reversible transition from low viscosity to high viscosity, so as to be pourable at one...
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5873991 |
Method for electrophoretic separation
Method for electrophoretic separation of nucleic acid fragments comprising the following steps: a) providing a polyacrylamide based gel prepared by photoinitiated polymerization of a mixture...
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5849166 |
Electrophoresis of nucleic acid fragments
A method for electrophoresis of nucleic acid fragments present in the solution which contains an amount, e.g., 0.2% or more, of a reagent, e.g., glycerol, dithiolthreitol (DTT) and trehalose or...
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5785832 |
Covalently cross-linked, mixed-bed agarose-polyacrylamide matrices for electrophoresis and chromatography
Covalently cross-linked, mixed-bed agarose-polyacrylamide matrices for electrophoresis and chromatography are disclosed. The mixed-bed matrices are formed by the copolymerization of a substituted...
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5759369 |
Viscous electrophoresis polymer medium and method
An electrophoresis separation medium is disclosed comprising a matrix of a copolymer composed of a linear hydrophilic polymer segment having a selected substantially uniform segment length and...
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5700365 |
Crosslinked polyacrylamide gels with high monomer: crosslinker ratios
Improved polyacrylamide-type gels utilizing diacrylyl tertiary amide crosslinkers are disclosed having relatively high monomer:crosslinker ratios in comparison to conventional polyacrylamide gels....
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5637202 |
Porous electrophoresis sponges
Porous plastic electrophoresis sponges having pore sizes range from 10 -9 to 10 -3 meters. The sponges are made from polyethylene, polypropylene, polytetrafluoroethylene, polyvinylidine...
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5589104 |
Electrophoresis separation gel and a method for preparing an electrophoresis separation gel
A gel for use in an electrophoresis separation includes a plurality of gradients within the gel. Each gradient includes a polyacrylamide matrix and a buffer, wherein the polyacrylamide of one...
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5578180 |
System for PH-neutral longlife precast electrophoresis gel
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5397449 |
Acrylic polymer electrophoresis support media
Electrophoresis support media which include one or more polymers made from hydroxy alkyl esters of acrylic or methylacrylic acid or poly (alkylene glycol) esters of acrylic or methacrylic acid....
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5354442 |
Matrix modification in the electrophoretic separation of nucleic acids
The present invention is a method for enhancing the separation of polynucleotides or polypeptides of various chain lengths or a mixture of polynucleotides and polypeptides of various chain lengths...
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5338428 |
Poly(N-Acylalkylenimine) electrophoresis support media
Electrophoresis support media which include one or more poly(N-Acylalkylenimine) polymers (PAEI). The polymers have the formula ##STR1## wherein m and p are between 1 and 3, n is between about 100...
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5319046 |
Polymers, and their use as gels for electrophoresis
A novel synthesis of N-acryloyl-tris-(hydroxymethyl)aminomethane (NAT) is described. The polymerization kinetics and transparency of the poly(NAT) gels crosslinked by a crosslinker (CL) e.g....
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5316638 |
Treatment of carbohydrates
Charged carbohydrate substances which have been run on an electrophoretic gel are transferred onto a porous membrane by a blotting technique such as an electrotransfer technique. This enables...
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