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7425254 |
***WITHDRAWN PATENT AS PER THE LATEST USPTO WITHDRAWN LIST*** Fractionation of macro-molecules using asymmetric pulsed field electrophoresis
A method and apparatus for fractionation of charged macro-molecules such as DNA is provided. DNA solution is loaded into a matrix including an array of obstacles. An alternating electric field...
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7347923 |
Dielectrophoresis device and method having insulating ridges for manipulating particles
Embodiments of the present invention provide methods and devices for manipulating particles using dielectrophoresis. Insulating ridges and valleys are used to generate a spatially non-uniform...
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7338808 |
Method and apparatus for determining zeta potential using alternating current electric field and T channel
Provided is a method for determining a zeta potential generated between a solid wall and a solution. The method includes (a) injecting an electrolyte solution into a first inlet of a T channel,...
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7282127 |
Microcapillary devices using high dielectric constant materials and related methods
A microcapillary device includes a microcapillary tube. An anode is positioned at a first end of the microcapillary tube. A cathode is positioned at a second end of said microcapillary tube. A...
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7282129 |
Traveling wave algorithms to focus and concentrate proteins in gel electrophoresis
An electrophoretic cell configuration and related method are disclosed that employ oppositely directed traveling electrical waves. The waves travel across the cell and samples undergoing...
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7189578 |
Methods and systems employing electrothermally induced flow for mixing and cleaning in microsystems
A method and system for controlling flow motion in a channel/cavity in a microfluidic system includes positioning at least one pair of electrodes in and/or proximate to the channel/cavity. A buffer...
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6939452 |
Parallel sample loading and injection device for multichannel microfluidic devices
A sample load and injection device ( 10 ) includes sample introduction capillaries ( 11 ) attached to a microfluidic device ( 12 ). Sample introduction capillaries ( 11 ) are connected to sample...
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6881317 |
Fractionation of macro-molecules using asymmetric pulsed field electrophoresis
A method and apparatus for fractionation of charged macro-molecules such as DNA is provided. DNA solution is loaded into a matrix including an array of obstacles. An alternating electric field...
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6866759 |
Stepped electrophoresis for movement and concentration of DNA
A fluidic channel patterned with a series of thin-film electrodes makes it possible to move and concentrate DNA in a fluid passing through the fluidic channel. The DNA has an inherent negative...
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6638408 |
Method and device for separation of charged molecules by solution isoelectric focusing
The invention provides a novel solution isoelectric focusing device and method that can reproducibly fractionate charged molecules into well-defined pools. This approach can be applied to mixtures...
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6444461 |
Microfluidic devices and methods for separation
Integrated systems, apparatus, software, and methods for performing biochemical analysis, including DNA sequencing, genomic screening, purification of nucleic acids and other biological components...
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5879528 |
Means and method of detection in chemical separation procedures
A means and method for indirect detection of constituent components of a mixture separated in a chemical separation process. Fluorescing ions are distributed across the area in which separation of...
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5846394 |
Method and apparatus for carrying out electrophoresis in fractal fields
A method and apparatus for carrying out electrophoretic separation is provided which imparts a fractal field component to the particles themselves or to the medium in which the particles are to be...
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5795455 |
Method for determining the nucleotide size of DNA fragments
A method for determining the nucleotide size of DNA fragments separated by gel electrophoresis, comprising the steps of (i) measuring the migration time of each detected DNA fragment, and (ii)...
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5759784 |
Method of nucleic acid transfer
The present invention is drawn to a method of dosaging a nucleic acid species comprising contacting a solid phase member or members, each member having a predetermined capacity of binding said...
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5667654 |
Real time in-gel assay
Methods are provided for assaying electrophoretically separated components of sample in a gel. Following an initial separation phase of the sample components into distinct bands within separate...
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5618701 |
Method of processing nucleic acid samples
A method of processing nucleic acid samples for analysis comprises the use of one or more manifolds (10) with a plurality of individual solid phase members (11) adapted for cooperation with a...
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5518604 |
Buffer shaping device
Electrophoresis apparatus with a gel support configured and arranged to hold a gel, a buffer chamber configured and arranged to hold a buffer, with the buffer contacting each end of the gel, and a...
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5468359 |
Method of determining presence of an analyte by isoelectric focusing
The separations resulting from capillary electrophoresis performed in a microbore capillary tube are detected on-line by focusing a light beam in the form of a line or sheet of light on the...
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5453162 |
Method and apparatus for gel electrophoresis using two electric fields
Disclosed is a method and apparatus for gel electrophoresis which simultaneously employs two or more electric fields in a single gel. According to this invention, which is defined as Multiple-Zone...
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5354442 |
Matrix modification in the electrophoretic separation of nucleic acids
The present invention is a method for enhancing the separation of polynucleotides or polypeptides of various chain lengths or a mixture of polynucleotides and polypeptides of various chain lengths...
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5348636 |
Processes for the preparation and separation of macromolecules
A process for the electrophoretic separation of charged macromolecules includes applying to the macromolecules a periodic sequence of pulses. Each period comprises a multiplicity of electric field...
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5336383 |
Pulsed field gel electrophoresis of large DNA
To separate high-molecular DNA strands, an electrophoresis system includes first means for changing the direction of an electric field within an electrophoresis separating unit at a first frequency...
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5294545 |
Concatemeric DNA length standards
The use of T7 bacteriophage to produce DNA length standards by enzymatically joining terminally repetitious, blunt-ended DNA has now been demonstrated. It is now possible to precisely control the...
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5178737 |
Electrophoretic resolution of single strand DNA by asymmetric field inversion
Single stranded DNA fragments in sizes above 300 bases are separated electrophoretically in a field inversion technique in which the voltage of the reverse pulse exceeds that of the forward pulse,...
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5176805 |
Reverse-polarity gel electrophoresis
Improved resolution of proteins and other macro molecules in gel electrophoresis is obtained by causing the molecules to move through the gel under the influence of an electrical potential, the...
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5167784 |
Sequencing large nucleic acid fragments
The base sequence of large single stranded nucleic acids is determined by retarding the migration rate of a nucleic acid fragment of n bases below a migration rate which would otherwise be the same...
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5135627 |
Mosaic microcolumns, slabs, and separation media for electrophoresis and chromatography
A method and compositions for separating molecules based on molecular size, shape, affinity, chirality, weight, charge, and hydrogen bonding, using a mosaic matrix formed by polymerizing a...
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5122248 |
Pulsed field capillary electrophoresis
Apparatus and method for conducting electrophoresis in a capillary tube under the influence of a pulsed electric field. Apparatus for separating and detecting molecular species of a sample in a...
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5108567 |
Electrophoresis method and apparatus with orthongonal field
A pulsed field electrophoresis method and apparatus for the separation of molecules wherein a fixed polarity potential difference between an anode and a cathode, disposed at respective opposed...
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5059294 |
Method for separating nucleic acids and nucleic acid probes
An improved method and device for nucleic acid hybridization assay employing combined direct an alternating field electrophoresis are disclosed. In the method of the present invention, a sample is...
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4995957 |
Device and method for the electrophoretic separation of macromolecules
An electrophoretic separation apparatus suitable for the separation of macromolecules is provided. Electrode elements are provided for generating an electric field for acting on a specimen....
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4971671 |
Processes for separation of DNA fragments
A process for enabling the separation of a mixture of DNA fragments comprising (1) providing an electrophoresis devive; (2) adding to the device a solution mixture containing DNA fragments of...
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4900416 |
Electrophoretic method
An electrophoretic method comprises the steps of positioning a sample, which contains nucleic acid fragments having different numbers of bases, in an electrophoresis medium composed of an...
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4886589 |
Electrophoresis method and apparatus for separating particles in a separation medium
Electrophoresis method and apparatus for separating particles in a separan medium (15) in which provided for driving the particles alternatively in different directions in the separation medium...
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4861448 |
Electrophoretic methods employing gel inserts
Gel inserts comprising a solidified liquid such as agarose suitable for use in an electrophoretic method, lysed cells entrapped within a matrix formed by the solidified liquid and macromolecules...
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4830726 |
Separation of large DNA molecules in alternating asymmetric electric fields
A method of separating DNA molecules by gel electrophoresis is disclosed which employs alternate application of high and low strength electric fields in opposite directions to a gel matrix...
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4740283 |
Pulsed-field gradient gel electrophoretic apparatus
A pulsed-field gradient gel electrophoretic apparatus is described wherein a free standing gel is employed. Electrode arrays are oriented to provide three dimensional fields across the face of the...
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4473452 |
Electrophoresis using alternating transverse electric fields
Disclosed are an apparatus for and a method of electrophoretically separating particles by electric fields which are transverse to each other, which alternate between respective high and low...
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3506554 |
APPARATUS FOR SEPARATING ELECTROPHORETICALLY ACTIVE SUBSTANCES
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