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US20120220492 Viral Vectors with Improved Properties  
Methods to improve the tropism or other features of a virus are disclosed. Such methods can be used to prepare, e.g., DNA or plasmid libraries of variants of a gene encoding a viral capsid or...
US20140179564 COMPOSITIONS AND METHODS FOR SELECTION OF NUCLEIC ACIDS CONTAINING MODIFIED BASES  
Methods are provided for reducing the complexity of a population of nucleic acids prior to performing an analysis of the nucleic acids, e.g., sequence analysis. The methods result in a subset of...
US20050130124 Phagemid display system  
The present invention provides a novel helper phage and phagemid and phagemid display system that comprises an amber mutation in gene 3 of the helper phage so that it is not expressed in the...
US20150045257 METHODS AND TRANSPOSON NUCLEIC ACIDS FOR GENERATING A DNA LIBRARY  
A method for the generation of DNA fragmentation library based on a transposition reaction in the presence of a transposon end with an engineered cleaveage site providing facilitated downstream...
US20130017978 METHODS AND TRANSPOSON NUCLEIC ACIDS FOR GENERATING A DNA LIBRARY  
A method for the generation of DNA fragmentation library based on a transposition reaction in the presence of a transposon end with an engineered cleaveage site providing facilitated downstream...
US20140243243 DEVICE AND METHOD FOR CELL-EXCLUSION PATTERNING  
This disclosure relates to devices and methods for cell-exclusion patterning. Specifically, this disclosure provides a device and method to exclude cells in selected areas during cell seeding and...
US20130281323 DEVICE AND METHOD FOR CELL-EXCLUSION PATTERNING  
This disclosure relates to devices and methods for cell-exclusion patterning. Specifically, this disclosure provides a device and method to exclude cells in selected areas during cell seeding and...
US20130017977 Microarray Synthesis and Assembly of Gene-Length Polynucleotides  
There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray...
US20120270750 Microarray Synthesis and Assembly of Gene-Length Polynucleotides  
There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray...
US20140323327 ANIMALS, REPERTOIRES & METHODS  
The present invention is directed to the concept of sectoring antibody gene segment repertoires in order to enable the development of novel, synthetic antibody chain repertoires not seen in...
US20120108440 REDUCING ADAPTER DIMER FORMATION  
Provided herein is a method of reducing adapter dimer formation comprising contacting a sample comprising target nucleic acid sequences with 5′ and 3′ adapters in the presence of one or more...
US20090023596 In Vitro Model Of Latent Mycobacterial Infection  
A method of inducing latency in Mycobacterium permits preparation of an in vitro model system of latent mycobacterial infection. Latency is induced in a pure culture of Mycobacterium by exposing...
US20140243213 Analysing Sequencing Bias  
The present invention relates to methods for determining the sequence bias of a sequencing technique. Furthermore, the invention relates to methods to reduce or enhance sequence bias during...
US20140309142 METHOD OF ON-CHIP NUCLEIC ACID MOLECULE SYNTHESIS  
A method of synthesizing a nucleic acid molecule, such as a gene, on a substrate or microchip is described. In particular, a method for synthesizing, amplifying, and assembling DNA...
US20110224102 Eukaryotic signal sequences for polypeptide expression and polypeptide display libraries  
The present invention generally relates to methods and compositions for expressing proteins or polypeptides in prokaryotic hosts using eukaryotic signal sequences.
US20150232918 Analyzing Messenger RNA and Micro RNA in the Same Reaction Mixture  
The present teachings provide methods, compositions, and kits for performing primer extension reactions on at least two target polynucleotides in the same reaction mixture. In some embodiments, a...
US20120184466 PARTHENOGENIC ACTIVATION OF HUMAN OOCYTES FOR THE PRODUCTION OF HUMAN EMBRYONIC STEM CELLS  
Methods of producing human stem cells are disclosed for parthenogenetically activating human oocytes by manipulation of O2 tension, including manipulation of Ca2+ under high O2 tension and...
US20120088677 METHODS AND COMPOSITIONS FOR ANALYSIS OF REGULATORY SEQUENCES  
Methods for constructing arrays of regulatory sequences, and the arrays so obtained, are provided. Regulatory sequences for use on the arrays are isolated based on their accessibility in cellular...
US20140329697 CONSTRUCTING METHOD OF HIGH-THROUGHPUT SEQUENCING LIBRARY AND USE THEREOF  
The present invention provides a method for constructing a high-throughput sequencing library, which comprises: fragmenting genomic DNA; end-repairing the DNA fragments; adding a base A to the 3′...
US20140235505 RNA ARRAY COMPOSITIONS AND METHODS  
Described herein are RNA arrays, and compositions and methods for generating RNA arrays, particularly high density RNA arrays. The disclosed methods for generating RNA arrays utilize template DNA...
US20130059761 Assembly of High Fidelity Polynucleotides  
Methods and apparatus relate to the synthesis of high fidelity polynucleotides and to the reduction of sequence errors generated during synthesis of nucleic acids on a solid support. Specifically,...
US20140200165 NOVEL FAB FRAGMENT LIBRARIES AND METHODS FOR THEIR USE  
The present invention provides Fab libraries and methods for using the Fab libraries to obtain antibodies against a target. The Fab library of the invention contains at least 109 different Fabs,...
US20120129731 Design and construction of diverse synthetic peptide and polypeptide libraries  
The present invention concerns the design and construction of diverse peptide and polypeptide libraries. In particular, the invention concerns methods of analytical database design for creating...
US20150051116 NEXT-GENERATION SEQUENCING LIBRARIES  
Provided herein is technology relating to next-generation sequencing and particularly, but not exclusively, to methods and compositions for preparing a next-generation sequencing library...
US20130157899 REAGENTS AND METHODS RELATING TO DNA ASSAYS USING AMPLICON PROBES ON ENCODED PARTICLES  
Encoded bead multiplex assays for chromosomal gains and losses are provided that provide the benefits of complex, large template DNA sources, such as BAC DNA, as the probe material without bead...
US20100298166 CELL POPULATIONS FOR POLYPEPTIDE ANALYSIS AND USES OF SAME  
Nucleic acid construct systems are disclosed. The constructs comprise: (i) a first nucleic acid construct comprising a first nucleic acid sequence encoding a first reporter polypeptide linked to...
US20090176654 Universal fibronectin type III binding-domain libraries  
Walk-through mutagenesis and natural-variant combinatorial fibronectin Type III (FN3) polypeptide libraries are described, along with their method of construction and use. Also disclosed are a...
US20140094385 SEQUENCE-SPECIFIC ENGINEERED RIBONUCLEASE H AND THE METHOD FOR DETERMINING THE SEQUENCE PREFERENCE OF DNA-RNA HYBRID BINDING PROTEINS  
The subject of the invention is the ribonuclease which cleaves RNA strand in DNA-RNA hybrids, wherein ribonuclease comprises fusion protein comprising catalytic domain of RNase HI (RNase HI) or...
US20130045894 Method for Amplification of Target Nucleic Acids Using a Multi-Primer Approach  
The present invention is a method for amplification of target nucleic acids wherein a first and second primer set is used to amplify a target nucleic acid in a single amplification reaction. The...
US20090054254 Method for Preparing Immunoglobulin Libraries  
The invention relates to the generation of immunoglobulin libraries and the identification and production of immunoglobulins having a specific functionality of interest.
US20150031855 Methods for Enhancing Bacterial Cell Display of Proteins and Peptides  
Methods of making and using bacterial display polypeptide libraries using circularly permuted OmpX (CPX) variants are disclosed. The invention further relates to methods for enhancing the display...
US20110152126 Process for Generating a Variant Library of DNA Sequences  
A method for generating a variant library of DNA sequences starting from at least one DNA starting sequence and including the following steps: a) selecting at least two mutation sites in the...
US20130338038 METHOD OF SCREENING COMPLEX PROTEIN LIBRARIES TO IDENTIFY ALTERED PROPERTIES  
The invention provides methods of making designed and constructed protein (e.g., antibody) libraries and libraries resulting from the same.
US20130338017 METHODS OF SCREENING COMPLEX PROTEIN LIBRARIES TO IDENTIFY ALTERED PROPERTIES  
The invention provides methods of making designed and constructed protein (e.g., antibody) libraries and libraries resulting from the same.
US20140057807 FIBRONECTIN CRADLE MOLECULES AND LIBRARIES THEREOF  
The here described invention discloses a combination of a top and bottom loop binder library using the CD and the FG loops of a number of FnIII domains (FnIII) (e.g., FnIII7, FnIII10 and FnIII14)...
US20140303000 COMPOSITIONS AND METHODS FOR DIRECTIONAL NUCLEIC ACID AMPLIFICATION AND SEQUENCING  
The invention provides methods and compositions, including kits, for directional nucleic acid amplification and sequencing. The invention further provides methods and compositions for the...
US20140243242 COMPOSITIONS AND METHODS FOR CO-AMPLIFYING SUBSEQUENCES OF A NUCLEIC ACID FRAGMENT SEQUENCE  
The present invention is related to genomic nucleotide sequencing. In particular, the invention describes a single reaction method to co-amplify multiple subsequences of a nucleic acid fragment...
US20130210681 Direct Cloning  
A method for performing homologous recombination between at least a first nucleic acid molecule and a second nucleic acid molecule which share at least one region of sequence homology. A method...
US20150152406 TRANSPOSITION-MEDIATED IDENTIFICATION OF SPECIFIC BINDING OR FUNCTIONAL PROTEINS  
The method disclosed herein describes a novel technology offering unparalleled efficiency, flexibility, utility and speed for the discovery and optimization of polypeptides having desired binding...
US20140128291 OLIGONUCLEOTIDES AND METHODS FOR THE PREPARATION OF RNA LIBRARIES  
Disclosed are compositions and methods for the preparation of RNA libraries for sequencing, gene expression profiling, microarray and other uses and for simplification of the library preparation...
US20120289426 METHOD FOR AMPLIFICATION OF TARGET NUCLEIC ACID  
A method for amplifying a target nucleic acid is disclosed, which includes: (a) fragmenting a nucleic acid sample to create a target fragment comprising a target nucleic acid and two...
US20100222237 Methods for Generating Polynucleotides Having Desired Characteristics by Iterative Selection and Recombination  
A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for...
US20140057799 System and Methods for Massively Parallel Analysis of Nucleic Acids in Single Cells  
Methods and systems are provided for massively parallel genetic analysis of single cells in emulsion droplets or reaction containers. Genetic loci of interest are targeted in a single cell using a...
US20150148246 SURFACE ANCHORED LIGHT CHAIN BAIT ANTIBODY DISPLAY SYSTEM  
The present invention provides, in part, an antibody display system that simultaneously uses a secretion and a display mode. Embodiments of the invention provide a system in which a bait complexed...
US20150111788 MULTIPLEX ISOLATION OF PROTEIN-ASSOCIATED NUCLEIC ACIDS  
The invention provides novel methods and materials for genetic and genomic analysis using single or multiplex isolation of protein-associated nucleic acids, including transposase-assisted...
US20140187447 Ligation Enhancement  
Compositions and methods are provided for enhancing enzymatic ligation between nucleic acid fragments that relies on one or more small molecule enhancers having a size of less than 1000 daltons....
US20120283144 Ligation Enhancement  
Compositions and methods are provided for enhancing enzymatic ligation between nucleic acid fragments that relies on one or more small molecule enhancers having a size of less than 1000 daltons....
US20140179561 HANGING DROP DEVICES, SYSTEMS AND/OR METHODS  
The present disclosure relates general to devices, systems, and methods of using such devices in creating and handling hanging drops of fluid. The present disclosure also relates to cell culture...
US20140200164 SYSTEM FOR DETECTION OF SPACED DROPLETS  
System, including methods and apparatus, for detection of spaced droplets.
US20120270740 POLONY SEQUENCING METHODS  
We describe ultra-high throughput polony genome sequencing that can permit, for example, generating raw data to re-sequencing the human genome in about one week (including library prep and...