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US20160001247 MICROARRAY SYNTHESIS AND ASSEMBLY OF GENE-LENGTH POLYNUCLEOTIDES  
There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray...
US20160000065 CRYOPRESERVATION OF CELLS AND SUBCELLULAR FRACTIONS  
The invention provides cryopreserved compositions of cells, wherein the compositions are advantageously in the form of self-sustaining bodies that can be individually handled and combined...
US20150376608 LIBRARY PREPARATION OF TAGGED NUCLEIC ACID USING SINGLE TUBE ADD-ON PROTOCOL  
A method of preparing a library of tagged nucleic acid fragments including contacting a population of cells directly with a lysis reagent having one or more protease to generate a cell lysate;...
US20150376607 AAV CAPSID PROTEINS FOR NUCLEIC ACID TRANSFER  
Recombinant adeno-associated viral (AAV) capsid proteins are provided. Methods for generating the recombinant adeno-associated viral capsid proteins and a library from which the capsids are...
US20150376581 T7 RNA POLYMERASE VARIANTS AND METHODS OF USING THE SAME  
The present invention relates to T7 RNA polymerase variants with improved affinity for 2′-modified nucleotides compared to the wildtype as well as methods for their production and methods of using...
US20150376575 PODOCYTE CULTURES AND USES THEREOF  
The following disclosure generally relates to methods of culturing podocytes in vitro. The cultures can be used for drug screening (such as medium or high throughput drug screening), and for...
US20150372182 RADIATION MICRODOSIMETERS CORRELATED WITH BIOLOGICAL CELLS AND CELL COMPONENTS  
One feature pertains to a radiation dosimeter comprising a microdosimeter cell array that includes a first microdosimeter cell having a first semiconductor volume configured to generate a first...
US20150368703 PRIMER TECHNOLOGY  
The present invention provides a method of nucleic acid manipulation comprising: (i) hybridizing a double stranded primer to the 3′ end of a single stranded nucleic acid template, wherein said...
US20150368687 Compositions and Methods for Synthesis of High Fidelity Oligonucleotides  
Aspects of the invention relate to methods, compositions for synthesizing high fidelity oligonucleotides.
US20150368639 COMPOSITIONS, METHODS AND USES FOR MULTIPLEX PROTEIN SEQUENCE ACTIVITY RELATIONSHIP MAPPING  
Embodiments herein concern systems, compositions, methods and uses for in vivo selection of optimum target proteins of use in designing genomically-engineered cells or organisms. Some embodiments...
US20150361481 MULTIPLEX NUCLEIC ACID AMPLIFICATION  
In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of...
US20150361423 HIGH THROUGHPUT GENE ASSEMBLY IN DROPLETS  
Provided herein is a method comprising: (a) obtaining a mixture of multiple sets of oligonucleotides, wherein the oligonucleotides within each set each comprise a terminal indexer sequence and can...
US20150361422 HIGH THROUGHPUT GENE ASSEMBLY IN DROPLETS  
Provided herein, among other things, is a method comprising: (a) obtaining a mixture of multiple sets of oligonucleotides, wherein the oligonucleotides within each set each comprise a terminal...
US20150360226 SINGLE CELL CAPTURE WITH POLYMER CAPTURE FILMS  
The present invention provides methods, systems, assemblies, and articles for capturing single cells with a polymer capture film. In certain embodiments, the polymer capture films comprise a...
US20150360195 DEVICE FOR RECOVERY AND ISOLATION OF BIOMOLECULES  
The present invention relates to a device for isolating and recovering a biomolecule from a test sample. The device includes a support and at least one peelable layer deposited on at least a...
US20150360194 Enrichment of DNA Sequencing Libraries from Samples Containing Small Amounts of Target DNA  
Provided herein is a method for capturing DNA molecules in solution. The method may comprise: extracting DNA from a sample that comprises endogenous DNA and environmental DNA to produce extracted...
US20150360193 COMPOSITIONS AND METHODS FOR THE AMPLIFICATION OF NUCLEIC ACIDS  
The present disclosure relates to systems and methods for the amplification of nucleic acids, including, but not limited to, the amplification of nucleic acid libraries and whole genome amplification.
US20150353994 BI-DIRECTIONAL SEQUENCING COMPOSITIONS AND METHODS  
In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support,...
US20150353993 COMPOSITIONS, METHODS, SYSTEMS AND KITS FOR TARGET NUCLEIC ACID ENRICHMENT  
The present invention provides methods, compositions, kits, systems and apparatus that are useful for isolating nucleic acid molecules from a sample. In particular, the methods generally relate to...
US20150353989 SAMPLE PREPARATION FOR NUCLEIC ACID AMPLIFICATION  
Presented are methods and compositions for preparing samples for amplification and sequencing. Particular embodiments relate to methods of obtaining nucleic acids material directly from tissues...
US20150353943 FUSION PROTEINS COMPRISING IMMUNOGLOBULIN CONSTANT DOMAIN-DERIVED SCAFFOLDS  
This disclosure features fusion proteins comprising a base protein linked to or incorporated in a CH2 scaffold of IgG. The CH2 scaffold can derive from the macaque CH2 domain of IgG. The fusion...
US20150353927 Templates, Libraries, Kits and Methods for Generating Molecules  
The present invention is directed to collections of templates for molecules such as RNA, as well as templates, devices, kits and methods for generating molecules from these collections. Through...
US20150353925 TRANSPOSON END COMPOSITIONS AND METHODS FOR MODIFYING NUCLEIC ACIDS  
The present invention provides methods, compositions and kits for using a transposase and a transposon end for generating extensive fragmentation and 5′-tagging of double-stranded target DNA in...
US20150353922 METHODS AND SYSTEMS FOR IDENTIFYING PATIENT SPECIFIC DRIVER MUTATIONS  
Methods for identifying patient specific driver mutations are provided. The methods provided identify specific patient derived markers associated with aberrant signal transduction pathways, in...
US20150353921 METHOD OF ON-CHIP NUCLEIC ACID MOLECULE SYNTHESIS  
A method of synthesizing a nucleic acid molecule, such as a gene, on a substrate or microchip is described. In particular, a method for synthesizing, amplifying, and assembling DNA...
US20150352512 BIOLOGICAL SAMPLE ANALYTICAL INSTRUMENT  
A method for processing a biological material sample includes dispensing a sample into wells of an array tape from a sample plate, dispensing a reagent into the wells of the array tape from a...
US20150346210 MULTIPLEX ASSAY FOR IMPROVED SCORING OF TUMOR TISSUES STAINED FOR PD-L1  
Multiplex assays for improved scoring of tumor tissues stained with PD-L1 featuring PD-L1 staining in a first color plus staining of one or more differentiating markers, such as a marker specific...
US20150344948 UNIVERSAL SANGER SEQUENCING FROM NEXT-GEN SEQUENCING AMPLICONS  
Described herein are methods, compositions and kits directed to amplification of nucleic acids suitable for both next generation sequencing (NGS) and a second round of sequencing as validation,...
US20150344943 METHODS AND SYSTEMS FOR NUCLEIC ACID AMPLIFICATION  
The disclosure provides methods and systems for nucleic acid amplification including isothermal nucleic acid amplification.
US20150344876 HIGH EFFICIENCY, SMALL VOLUME NUCLEIC ACID SYNTHESIS  
The disclosure generally relates to compositions and methods for the production of nucleic acid molecules. In some aspects, the invention allows for the microscale generation of nucleic acid...
US20150344875 Isolating Biological Modulators from Biodiverse Gene Fragment Libraries  
The present invention provides a method for identifying a modulator or mediator of a biological activity, which activity includes antigenicity and or immunogenicity, said method comprising the...
US20150344873 Whole Genome Mapping by DNA Sequencing With Linked-Paired-End Library  
The present invention relates to innovative means of generating sequence-linked DNA fragments and subsequent uses of such linked DNA fragments for de novo haplotype-resolved whole genome mapping...
US20150344871 System and Methods for Massively Parallel Analysis of Nucleic Acids in Single Cells  
Methods and systems are provided for massively parallel genetic analysis of single cells in emulsion droplets or reaction containers. Genetic loci of interest are targeted in a single cell using a...
US20150343410 Preparation of Templates for Nucleic Acid Sequencing  
The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands...
US20150337368 METHODS AND PRIMER SETS FOR HIGH THROUGHPUT PCR SEQUENCING  
The invention relates to a method for amplifying a target nucleic acid sequence tn-tC-tV-tC′-tn′ comprising a first amplification using a first primer pair with sequence ma-K-pC and ma-K′-pC′, and...
US20150337351 METHODS OF MICROORGANISM IMMOBILIZATION  
The present disclosure is related to methods for immobilizing microorganisms to produce an immobilized microorganism sample for detection with a detection system. Compositions for immobilizing...
US20150337298 HAPLOIDOME DETERMINATION BY DIGITIZED TRANSPOSONS  
In certain embodiments, the present invention provides a way of “digitally” marking different the alleles of different chromosomes by using a transposase to insert differently barcoded transposons...
US20150337296 Arrays and Methods of Use  
Methods are provided for producing a molecular array comprising a plurality of molecules immoblised to a solid substrate at a density which allows individual immobilised molecules to be...
US20150337295 INTEGRATED SINGLE CELL SEQUENCING  
This disclosure provides a method of forming tagged nucleic acid sequences. A target polynucleotide is immobilized on a solid support; a recognition-oligonucleotide is hybridized thereto; the...
US20150337292 METHOD OF PRODUCING SECRETABLE ANTIBODIES BY EXPRESSION IN SACCHAROMYCES CEREVISIAE  
The invention relates to a method for the production and non-covalent surface display of antibodies and derived fragments as well as molecule libraries based thereon on the surface of S....
US20150337290 METHODS AND PRODUCTS FOR MUTATING NUCLEOTIDE SEQUENCES  
Described herein are products and methods directed to introducing deletions, insertions and substitutions into nucleotide sequences using transposons. Described herein are isolated transposons...
US20150329856 METHODS AND SYSTEMS FOR THE GENERATION OF A PLURALITY OF SECURITY MARKERS AND THE DETECTION THEROF  
This invention pertains to methods for generating large quantities of DNA security markers by combinatorial variation techniques using polymorphic fragment length DNA for unique identification...
US20150329855 AMPLIFICATION PRIMERS AND METHODS  
The present invention provides methods, compositions, and kits for performing amplification (e.g., whole genome amplification) employing primers that have a 5′ restriction site, a 3′ random...
US20150329853 COMPOSITIONS AND METHODS FOR CREATING ALTERED AND IMPROVED CELLS AND ORGANISMS  
The present invention provides compositions comprising randomized in-frame fusion polynucleotides and methods for introducing them into a host organism to identify desirable phenotypic changes...
US20150322503 Reagents and Methods of PCR  
Modified double-stranded oligonucleotides that have terminal regions on each of their strands, that have a hybrid length of 6-50 nucleotides long, that have a melting temperature Tm of at least...
US20150322490 METHODS AND COMPOSITIONS FOR MULTIPLEX PCR  
The present invention provides methods, compositions, kits, systems and apparatus that are useful for multiplex PCR of one or more nucleic acids present in a sample. In particular, various...
US20150315631 MICROFLUIDIC SYSTEM FOR AMPLIFYING AND DETECTING POLYNUCLEOTIDES IN PARALLEL  
The present technology provides for an apparatus for detecting polynucleotides in samples, particularly from biological samples. The technology more particularly relates to microfluidic systems...
US20150315571 Method of Nucleic Acid Fragmentation  
A method of preparing an at least partially randomly sheared library of nucleic acids is provided. The method includes the steps of providing a source of nucleic acids, randomly incorporating...
US20150315570 DIRECTED EVOLUTION OF SYNTHETIC GENE CLUSTER  
The invention relates to methods and products for generating diverse libraries of genetic material. The products include libraries and constructed nucleic acids as well as kits and databases and...
US20150315554 CELL LINES EXPRESSING CFTR AND METHODS OF USING THEM  
Disclosed herein are cells and cell lines that stably express CFTR and methods for using those cells and cell lines. The invention also includes techniques for creating these cells and cell lines....